Induced chirality of binary aggregates of oppositely charged water-soluble porphyrins on DNA matrix
文献类型：期刊论文
| 作者 | Chen, XD; Liu, MH |
| 刊名 | JOURNAL OF INORGANIC BIOCHEMISTRY
 |
| 出版日期 | 2003-02-01 |
| 卷号 | 94期号:1-2页码:106-113 |
| 关键词 | Induced Chirality Dna Porphyrin Resonance Light Scattering |
| ISSN号 | 0162-0134 |
| DOI | 10.1016/S0162-0134(02)00645-1 |
| 英文摘要 | The induced chirality of achiral binary aggregates of meso-tetrakis(4-N-methylpyridyl)porphyrine (TMPyP) and meso-tetrakis(4-sulfonatophenyl)porphyrine (TPPS) on a deoxyribonucleic acid (DNA) matrix was investigated. Although the negatively charged TPPS did not show induced chirality in DNA solution due to the electrostatic repulsion, induced chirality was obtained through the addition of a positively charged TMPyP. It was confirmed that the induced chirality was due to the binary complex formation between TPPS and TMPyP on the DNA matrix. Moreover, the induced chirality depended on the relative molar ratio of TPPS to TMPyP (r) and the binding modes of the complex to DNA. When r<1, induced circular dichroism (CD) spectrum of the ternary complex was similar to that of intercalated TMPyP into DNA. For r=1, the induced CD spectrum showed a reversed biphasic signal due to the complex of TMPyP and TPPS stacking along the DNA surface. At a higher r value (>1), there was an induced CD signal at 482 nm attributed to a lateral shifted arrangement of heteroaggregate of TPPS and TMPyP on DNA matrix where TMPyP acted as a spacer to mediate the growth of heteroaggregates. Increasing the concentration of sodium chloride in the solution would favor the formation of the lateral shifted arrangement of heteroaggregate of TPPS and TMPyP The resonance light scattering (RLS) spectra confirmed the above results. Analysis of the CD spectral changes in DNA conformation showed that during the binary complex formation of TPPS and TMPyP, the intercalated TMPyP could be 'pulled out' from the base pairs of DNA, which might be useful in gene therapy. A model was proposed to account for these observations. (C) 2002 Elsevier Science Inc. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000181499700014 |
| 出版者 | ELSEVIER SCIENCE INC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/81029]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, MH |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Lab Colloid & Interface Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, XD,Liu, MH. Induced chirality of binary aggregates of oppositely charged water-soluble porphyrins on DNA matrix[J]. JOURNAL OF INORGANIC BIOCHEMISTRY,2003,94(1-2):106-113. |
| APA | Chen, XD,&Liu, MH.(2003).Induced chirality of binary aggregates of oppositely charged water-soluble porphyrins on DNA matrix.JOURNAL OF INORGANIC BIOCHEMISTRY,94(1-2),106-113. |
| MLA | Chen, XD,et al."Induced chirality of binary aggregates of oppositely charged water-soluble porphyrins on DNA matrix".JOURNAL OF INORGANIC BIOCHEMISTRY 94.1-2(2003):106-113. |
入库方式： OAI收割
来源：化学研究所
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