Direct observations of the cleavage-reaction of an L-DPPC monolayer catalyzed by phospholipase A(2) and inhibited by an indole inhibitor at the air/water interface
文献类型：期刊论文
| 作者 | Zhai, XH; Li, JB; Brezesinski, G; He, Q; Mohwald, H; Lai, LH; Liu, Y; Liu, L; Gao, Y |
| 刊名 | CHEMBIOCHEM
 |
| 出版日期 | 2003-04-04 |
| 卷号 | 4期号:4页码:299-305 |
| 关键词 | Brewster Angle Microscopy Enzymes Hydrolysis Inhibitors Phospholipids |
| ISSN号 | 1439-4227 |
| 英文摘要 | The enzymatic hydrolysis of an L-dipalmitoylphoisphatidylcholine (L-DPPC) monolayer at the air/water interface, catalyzed by phospholipase A(2) (PLA(2)), serves as a model for biospecific interfacial reactions. The cleavage of L-DPPC was investigated by Brewster angle microscopy. Different types of domain defects were observed to form in the coexisting liquid expanded and liquid condensed phases during the hydrolysis reaction. The adsorption of the enzyme was quantitatively recorded as the increase of the surface pressure over a fixed molecular area with time. In the case of the following processes: adsorption of PLA(2), cleavage reaction, and rearrangement of substrate and product molecules at the interface. Addition of a PLA(2) inhibitor to the lipid monolayer leads to a fast surface pressure increase after enzyme injection. The surface pressure reaches a maximum value and then does not change for a long time. During this period, no change in the domain shape and number density was observed, which indicates that the enzyme is inhibited for a certain period of time. The experimental results provide the possibility of a direct way to prove inhibitor activity. |
| 语种 | 英语 |
| WOS记录号 | WOS:000182220400007 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/81169]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, JB |
| 作者单位 | 1.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Int Joint Lab, Beijing 100080, Peoples R China 2.Max Planck Inst Colloids & Interfaces, D-14476 Golm Potsdam, Germany 3.Peking Univ, Inst Phys Chem, Coll Chem & Mol Engn, Beijing 100871, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhai, XH,Li, JB,Brezesinski, G,et al. Direct observations of the cleavage-reaction of an L-DPPC monolayer catalyzed by phospholipase A(2) and inhibited by an indole inhibitor at the air/water interface[J]. CHEMBIOCHEM,2003,4(4):299-305. |
| APA | Zhai, XH.,Li, JB.,Brezesinski, G.,He, Q.,Mohwald, H.,...&Gao, Y.(2003).Direct observations of the cleavage-reaction of an L-DPPC monolayer catalyzed by phospholipase A(2) and inhibited by an indole inhibitor at the air/water interface.CHEMBIOCHEM,4(4),299-305. |
| MLA | Zhai, XH,et al."Direct observations of the cleavage-reaction of an L-DPPC monolayer catalyzed by phospholipase A(2) and inhibited by an indole inhibitor at the air/water interface".CHEMBIOCHEM 4.4(2003):299-305. |
入库方式： OAI收割
来源：化学研究所
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