Dynamic and morphological investigation of phospholipid monolayer hydrolysis by phospholipase C
文献类型：期刊论文
| 作者 | He, Q; Li, JB |
| 刊名 | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
 |
| 出版日期 | 2003-01-10 |
| 卷号 | 300期号:2页码:541-545 |
| 关键词 | Brewster Angle Microscopy Hydrolysis Phospholipase c Phospholipid Monolayer |
| ISSN号 | 0006-291X |
| DOI | 10.1016/S0006-291X(02)02893-0 |
| 英文摘要 | The hydrolytic reaction of L-alpha-dipalmitoylphosphatidylcholine (L-DPPC) monolayer catalyzed by phospholipase C (PLC) has been investigated using monomolecular membrane technique and Brewster angle microscopy (BAM) at the air/water interface. The curves of surface pressure as a function of time determined a lag-burst process of L-DPPC monolayer hydrolysis by PLC. The BAM images recorded the changes of domains formed in the coexistence region of liquid-condensed (LC) and liquid-expanded (LE) phases during the monolayer hydrolysis. The changes of domain shape and size and the increase of domain number reflect the decrease of reactant and molecular rearrangement process of the main hydrolysis product, dipalmitoylglycerol (DPG). A new phase was observed after the hydrolysis reaction was completed. (C) 2002 Elsevier Science (USA). All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000180607000043 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/81391]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Li, JB |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Int Joint Lab, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Q,Li, JB. Dynamic and morphological investigation of phospholipid monolayer hydrolysis by phospholipase C[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2003,300(2):541-545. |
| APA | He, Q,&Li, JB.(2003).Dynamic and morphological investigation of phospholipid monolayer hydrolysis by phospholipase C.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,300(2),541-545. |
| MLA | He, Q,et al."Dynamic and morphological investigation of phospholipid monolayer hydrolysis by phospholipase C".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 300.2(2003):541-545. |
入库方式： OAI收割
来源：化学研究所
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