Real time kinetic analysis of the interaction between immunoglobulin G and histidine using quartz crystal microbalance biosensor in solution
文献类型：期刊论文
| 作者 | Liu, Y; Yu, X; Zhao, R; Shangguan, DH; Bo, ZY; Liu, GQ |
| 刊名 | BIOSENSORS & BIOELECTRONICS
 |
| 出版日期 | 2003-10-01 |
| 卷号 | 18期号:11页码:1419-1427 |
| 关键词 | Piezoelectric Biosensor Flow Injection Analysis Kinetic Affinity Constant Immunoglobulin g Histidine |
| ISSN号 | 0956-5663 |
| DOI | 10.1016/S0956-5663(03)00090-3 |
| 英文摘要 | Quartz crystal microbalance (QCM) biosensor integrated in a flow injection analysis (FIA) system was used for the investigation of the specific interaction between immunoglobin G (IgG) and histidine. The histidine was immobilized on the gold electrodes of the piezoelectric crystal using appropriate procedures based on self-assembling of the dithiothreitol (DTT). The specific interaction of the immobilized ligand with IgG in solution was followed as a change in the resonant frequency of the modified crystal and studied in real time without any additional labels. With the mass sensitive biosensor system, the differences in affinity of three different species of IgG: human IgG, goat IgG and mouse IgG were easily distinguished and their respective kinetic rate constants (k(ass) and k(diss)) and equilibrium association constants (K-A) were determined from the curves of frequency versus time. For the interactions, K-A were 2.92 x 10(4), 3.23 x 10(4) and 4.08 x 10(4) M-1 for human IgG, goat IgG and mouse IgG, respectively. (C) 2003 Elsevier Science B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000185193500014 |
| 出版者 | ELSEVIER ADVANCED TECHNOLOGY |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/81397]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Liu, GQ |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Y,Yu, X,Zhao, R,et al. Real time kinetic analysis of the interaction between immunoglobulin G and histidine using quartz crystal microbalance biosensor in solution[J]. BIOSENSORS & BIOELECTRONICS,2003,18(11):1419-1427. |
| APA | Liu, Y,Yu, X,Zhao, R,Shangguan, DH,Bo, ZY,&Liu, GQ.(2003).Real time kinetic analysis of the interaction between immunoglobulin G and histidine using quartz crystal microbalance biosensor in solution.BIOSENSORS & BIOELECTRONICS,18(11),1419-1427. |
| MLA | Liu, Y,et al."Real time kinetic analysis of the interaction between immunoglobulin G and histidine using quartz crystal microbalance biosensor in solution".BIOSENSORS & BIOELECTRONICS 18.11(2003):1419-1427. |
入库方式： OAI收割
来源：化学研究所
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