Direct chemiluminescence determination of cysteine in human serum using quinine-Ce(IV) system
文献类型：期刊论文
| 作者 | Nie, LH; Ma, HM; Sun, M; Li, XH; Su, MH; Liang, SC |
| 刊名 | TALANTA
 |
| 出版日期 | 2003-04-10 |
| 卷号 | 59期号:5页码:959-964 |
| 关键词 | Chemiluminescence Cysteine Quinine-ce(Iv) System Human Serum |
| ISSN号 | 0039-9140 |
| DOI | 10.1016/S0039-9140(02)00649-5 |
| 英文摘要 | A simple, sensitive and selective chemiluminescence (CL) method was developed for the determination of cysteine. This method is based on that the weak CL of cysteine oxidized with cerium (IV) can be greatly enhanced by quinine. The calibration curve was linear over the range 3.5 x 10(-9)-3.5 x 10(-6) M with a detection limit of 2.5 x 10(-9) M (S/N N = 3). The RSD was found to be 8.4% by 10 replicate determinations of 2.9 x 10(-8) M cysteine. Due to high sensitivity, the proposed method can be used directly to determine the total concentration of cysteine in human serum through simply diluting the sample for a thousand fold. The obtained result was in agreement with that given by amino acid autoanalyzer. The present method does not require any separation, showing a simpler analytical characteristic. The mechanism of the CL reaction was also discussed. (C) 2003 Elsevier Science B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000182494500013 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/81483]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Ma, HM |
| 作者单位 | Chinese Acad Sci, Ctr Mol Sci, Biol Chem Lab, Inst Chem, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Nie, LH,Ma, HM,Sun, M,et al. Direct chemiluminescence determination of cysteine in human serum using quinine-Ce(IV) system[J]. TALANTA,2003,59(5):959-964. |
| APA | Nie, LH,Ma, HM,Sun, M,Li, XH,Su, MH,&Liang, SC.(2003).Direct chemiluminescence determination of cysteine in human serum using quinine-Ce(IV) system.TALANTA,59(5),959-964. |
| MLA | Nie, LH,et al."Direct chemiluminescence determination of cysteine in human serum using quinine-Ce(IV) system".TALANTA 59.5(2003):959-964. |
入库方式： OAI收割
来源：化学研究所
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