Chromatic immunoassay based on polydiacetylene vesicles
文献类型：期刊论文
| 作者 | Su, YL; Li, JR; Jiang, L |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2004-10-10 |
| 卷号 | 38期号:1-2页码:29-33 |
| 关键词 | Chromatic Immunoassay Polydiacetylene Vesicles |
| ISSN号 | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2004.08.010 |
| 英文摘要 | A new approach of chromatic immunoassay based on polydiacetylene vesicles is described. Antibodies were covalently coupled with mixed vesicles of 40,12-tricosadiynoic acid (TCDA) and dimyristoylphosphaticlycholine (DMPC). The vesicle-antibody conjugates were irradiated with UV light to yield a blue-colored polydiacetylene. After antigen injection, specific immunoreactions took place at the vesicle surface alter polydiacetylene conformation and lead to a color change from blue to red. The chromatic immunoassay described here is simple, rapid, sensitive; the color change was readily discernible by naked eye when the concentration of antigen is 1 ng/mL. Incorporation of DMPC in the mixed vesicles increases the sensitivity of the chromatic immunoassay. (C) 2004 Elsevier B.V. All rights reserved. |
| 语种 | 英语 |
| WOS记录号 | WOS:000224672800005 |
| 出版者 | ELSEVIER SCIENCE BV |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/81629]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Jiang, L |
| 作者单位 | Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Key Lab Colloid & Interface Sci, Beijing 100080, Peoples R China |
| 推荐引用方式 GB/T 7714 | Su, YL,Li, JR,Jiang, L. Chromatic immunoassay based on polydiacetylene vesicles[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2004,38(1-2):29-33. |
| APA | Su, YL,Li, JR,&Jiang, L.(2004).Chromatic immunoassay based on polydiacetylene vesicles.COLLOIDS AND SURFACES B-BIOINTERFACES,38(1-2),29-33. |
| MLA | Su, YL,et al."Chromatic immunoassay based on polydiacetylene vesicles".COLLOIDS AND SURFACES B-BIOINTERFACES 38.1-2(2004):29-33. |
入库方式： OAI收割
来源：化学研究所
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