Chiral separation and enantiomeric purity determination of pazufloxacin mesilate by HPLC using chiral mobile phase additives
文献类型：期刊论文
| 作者 | Zhang, ZF; Yang, GL; Wang, DX; Liang, GJ; Chen, Y |
| 刊名 | JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES
 |
| 出版日期 | 2004 |
| 卷号 | 27期号:5页码:813-827 |
| 关键词 | Chiral Mobile Phase Additives Chiral Separation Phase L-phenylalanine R-and S-enantiomers |
| ISSN号 | 1082-6076 |
| DOI | 10.1081/JLC-120029700 |
| 英文摘要 | In this work, a chiral separation method based on an ODS column and chiral mobile phase additives (CMPA) for a new fluoroquinolones drug pazufloxacin mesilate [(-)-(S)-10-(1-amino-cyclopropyl)-9-fluoro-3-methyl-7-oxo-2,3-dihydro-7H-pyrido(1,2,3-de)-1,4-benzoxazine-6-carboxylic acid mesilate] and its R-(+)-enantiomer was developed. The chiral separation was performed on a Shimadzu CLC-ODS column (150 x 6.3 mm, 10 mum), methanol-phenylalanine solution (containing 8 mmol L-1 L-phenylalanine and 6.3 mmol L-1 copper sulfate, adjusted to pH = 3.5 with sodium hydroxide solution) (30: 70) was used as the chiral mobile phase and detected at 332 nm. Under the above conditions, the two enantiomers could be baseline separated with a resolution 1.9 and the chiral purity was quantitatively determined. Calibration graphs were constructed in the range of 2.48-248 mug mL(-1) for S-(-)- and 2.52-252 mug mL(-1) for R-(+)-enantiomer, respectively. The linear correlation equations were: Y = 1.38 x 10(5)X - 130.9 (r = 0.9998) for S-(-)- and Y = 1.26 x 10(5)X - 60.6 (r = 0.9998) for R-(+) enantiomer, respectively. The detection limits were 1.2 ng for S-(-)- and 1.5 ng for R-(+)- enantiomer(obtained by SIN = 3), respectively. The RSD of the method was below 1.5% (n = 5). |
| 语种 | 英语 |
| WOS记录号 | WOS:000220591700004 |
| 出版者 | MARCEL DEKKER INC |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/82567]  |
| 专题 | 中国科学院化学研究所 |
| 通讯作者 | Yang, GL |
| 作者单位 | 1.Hebei Univ, Coll Chem & Environm Sci, Baoding 071002, Peoples R China 2.Chinese Acad Sci, Inst Chem, Ctr Mol Sci, Beijing 100080, Peoples R China 3.Gen Hosp AF PLA, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, ZF,Yang, GL,Wang, DX,et al. Chiral separation and enantiomeric purity determination of pazufloxacin mesilate by HPLC using chiral mobile phase additives[J]. JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,2004,27(5):813-827. |
| APA | Zhang, ZF,Yang, GL,Wang, DX,Liang, GJ,&Chen, Y.(2004).Chiral separation and enantiomeric purity determination of pazufloxacin mesilate by HPLC using chiral mobile phase additives.JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES,27(5),813-827. |
| MLA | Zhang, ZF,et al."Chiral separation and enantiomeric purity determination of pazufloxacin mesilate by HPLC using chiral mobile phase additives".JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES 27.5(2004):813-827. |
入库方式： OAI收割
来源：化学研究所
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