Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe
文献类型：期刊论文
| 作者 | Zhang, Suge1,2; Sun, Hongxia1; Wang, Lixia1; Liu, Yan1; Chen, Hongbo1,2; Li, Qian1; Guan, Aijiao1; Liu, Meirong3; Tang, Yalin1,2 |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2018-09-06 |
| 卷号 | 46期号:15页码:7522-7532 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gky665 |
| 英文摘要 | G-quadruplex DNA has been viewed as a prospective anti-cancer target owing to its potential biological relevance. Real-time monitoring of DNA G-quadruplex structures in living cells can provide valuable insights into the relationship between G-quadruplex formation and its cellular consequences. However, the probes capable of detecting DNA G-quadruplexes in living cells are still very limited. Herein, we reported a new fluorescent probe, IMT, for real-time visualization of DNA G-quadruplex structures in living cells. Using IMT as a fluorescent indicator, the quantity changes of DNA G-quadruplex at different points in time during continuous cellular progression responding to Aphidicolin and Hydroxyurea treatment have been directly visualized. Our data demonstrate that IMT will be a valuable tool for exploring DNA G-quadruplexes in live cells. Further application of IMT in fluorescence imaging may reveal more information on the roles of DNA G-quadruplexes in biological systems. |
| 语种 | 英语 |
| WOS记录号 | WOS:000444148100011 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://ir.iccas.ac.cn/handle/121111/41890]  |
| 专题 | 化学研究所_分子动态与稳态结构实验室 |
| 通讯作者 | Sun, Hongxia; Tang, Yalin |
| 作者单位 | 1.Chinese Acad Sci, Beijing Natl Lab Mol Sci, State Key Lab Struct Chem Unstable & Stable Speci, CAS Res Educ Ctr Excellence Mol Sci,Inst Chem, Beijing 100190, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Ctr Physiochem Anal & Measurement, Inst Chem, Beijing 100190, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Suge,Sun, Hongxia,Wang, Lixia,et al. Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe[J]. NUCLEIC ACIDS RESEARCH,2018,46(15):7522-7532. |
| APA | Zhang, Suge.,Sun, Hongxia.,Wang, Lixia.,Liu, Yan.,Chen, Hongbo.,...&Tang, Yalin.(2018).Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe.NUCLEIC ACIDS RESEARCH,46(15),7522-7532. |
| MLA | Zhang, Suge,et al."Real-time monitoring of DNA G-quadruplexes in living cells with a small-molecule fluorescent probe".NUCLEIC ACIDS RESEARCH 46.15(2018):7522-7532. |
入库方式： OAI收割
来源：化学研究所
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