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Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealedby Lifetime Labeling of Germline

文献类型：期刊论文

作者Ye, Ding1,2; Zhu, Lin1,2; Zhang, Qifeng1,2; Xiong, Feng1; Wang, Houpeng1; Wang, Xiaosi1,2; He, Mudan1,2; Zhu, Zuoyan1,2; Sun, Yonghua1,2
刊名MARINE BIOTECHNOLOGY
出版日期2019-04-01
卷号21期号:2页码:217-228
ISSN号1436-2228
关键词piwil1 Spermatogenesis Oogenesis Primordial germ cell Spermatogonia Oogonia
DOI10.1007/s10126-019-09874-1
通讯作者Sun, Yonghua(yhsun@ihb.ac.cn)
英文摘要Teleost sex differentiation largely depends on the number of undifferentiated germ cells. Here, we describe the generation and characterization of a novel transgenic zebrafish line, Tg(piwil1:egfp-UTRnanos3)(ihb327Tg), which specifically labels the whole lifetime of germ cells, i.e., from primordial germ cells (PGCs) at shield stage to the oogonia and early stage of oocytes in the ovary and to the early stage of spermatogonia, spermatocyte, and spermatid in the testis. By using this transgenic line, we carefully observed the numbers of PGCs from early embryonic stage to juvenile stage and the differentiation process of ovary and testis. The numbers of PGCs became variable at as early as 1day post-fertilization (dpf). Interestingly, the embryos with a high amount of PGCs mainly developed into females and the ones with a low amount of PGCs mainly developed into males. By using transient overexpression and transgenic induction of PGC-specific bucky ball (buc), we further proved that induction of abundant PGCs at embryonic stage promoted later ovary differentiation and female development. Taken together, we generate an ideal transgenic line Tg(piwil1:egfp-UTRnanos3)(ihb327Tg) which can visualize zebrafish germline for a lifetime, and we have utilized this line to study germ cell development and gonad differentiation of teleost and to demonstrate that the increase of PGC number at embryonic stage promotes female differentiation.
WOS关键词SEX-DIFFERENTIATION ; GENE-EXPRESSION ; MAINTENANCE ; TRANSITION ; DIMORPHISM ; TRANSGENE ; MIGRATION ; DEPLETION ; KNOCKOUT ; PATTERN
资助项目National Key R&D Program of China[2018YFD0901205] ; National Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31872550] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA08010106] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ03] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2015FB11]
WOS研究方向Biotechnology & Applied Microbiology ; Marine & Freshwater Biology
语种英语
出版者SPRINGER
WOS记录号WOS:000462935700009
资助机构National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; Strategic Priority Research Program of Chinese Academy of Sciences ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology
源URL[http://ir.ihb.ac.cn/handle/342005/27837]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心
水生生物研究所_水生生物多样性与资源保护研究中心
通讯作者Sun, Yonghua
作者单位1.Chinese Acad Sci, Innovat Acad Seed Design, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Coll Adv Agr Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Ye, Ding,Zhu, Lin,Zhang, Qifeng,et al. Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealedby Lifetime Labeling of Germline[J]. MARINE BIOTECHNOLOGY,2019,21(2):217-228.
APA Ye, Ding.,Zhu, Lin.,Zhang, Qifeng.,Xiong, Feng.,Wang, Houpeng.,...&Sun, Yonghua.(2019).Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealedby Lifetime Labeling of Germline.MARINE BIOTECHNOLOGY,21(2),217-228.
MLA Ye, Ding,et al."Abundance of Early Embryonic Primordial Germ Cells Promotes Zebrafish Female Differentiation as Revealedby Lifetime Labeling of Germline".MARINE BIOTECHNOLOGY 21.2(2019):217-228.

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来源：水生生物研究所

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