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A simplified method for the simultaneous detection of nervous necrosis virus and iridovirus in grouper Epinephelus spp.

文献类型：期刊论文

作者Yu, N-T1,2; Zhang, Y-L1; Xiong, Z.3,4; Qu, L.1; Liu, Z-X1
刊名ACTA VIROLOGICA
出版日期2019
卷号63期号:1页码:80-87
关键词duplex reverse transcription-PCR nervous necrosis virus iridovirus grouper
ISSN号0001-723X
DOI10.4149/av_2019_107
通讯作者Yu, N-T(yunaitong@163.com) ; Liu, Z-X(liuzhixin@itbb.org.cn)
英文摘要Grouper nervous necrosis virus (GNNV) and grouper iridovirus (GIV) are major grouper-infecting viruses in southern China that can cause serious economic losses. A duplex reverse transcription-PCR (duplex RT-PCR) method was developed for the simultaneous detection of GNNV and GIV. Eight groups of primers specifically targeting the capsid protein genes of GNNV and GIV were designed and analyzed. The primer set GN4 was selected and used to amplify fragments of 887 bp and 319 bp in length from GNNV and GIV, respectively. Furthermore, the duplex PCR assay was shown to be sensitive because it could detect at least 20 pg of plasmid-viral DNA from a mixture of viruses. Using this assay, 18 GNNV infected groupers and 7 GIV infected groupers were detected amongst 41 suspected samples in Hainan. The duplex RT-PCR assay proved to be a rapid, specific, and sensitive method for detecting the two grouper viruses. This method could be used to facilitate better control of fish viruses through early detection.
WOS关键词MULTIPLEX RT-PCR ; SPOTTED GROUPER ; BETANODAVIRUS ; PATHOGENICITY ; MONOPLEX ; SJNNV
资助项目National Nonprofit Institute Research Grant of CATAS[1630052016014] ; Major Science and Technology Program of Hainan Province[ZDKJ2017003] ; Young Elite Scientists Sponsorship Program by CSTC[CSTC-QN201704]
WOS研究方向Virology
语种英语
WOS记录号WOS:000468126700009
出版者AEPRESS SRO
资助机构National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; National Nonprofit Institute Research Grant of CATAS ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Major Science and Technology Program of Hainan Province ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC ; Young Elite Scientists Sponsorship Program by CSTC
源URL[http://ir.ihb.ac.cn/handle/342005/28103]  
专题水生生物研究所_其他
通讯作者Yu, N-T; Liu, Z-X
作者单位1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Univ Arizona, Sch Plant Sci, Tucson, AZ 85721 USA
4.Univ Arizona, BIO5 Inst, Tucson, AZ 85721 USA
推荐引用方式
GB/T 7714		 Yu, N-T,Zhang, Y-L,Xiong, Z.,et al. A simplified method for the simultaneous detection of nervous necrosis virus and iridovirus in grouper Epinephelus spp.[J]. ACTA VIROLOGICA,2019,63(1):80-87.
APA Yu, N-T,Zhang, Y-L,Xiong, Z.,Qu, L.,&Liu, Z-X.(2019).A simplified method for the simultaneous detection of nervous necrosis virus and iridovirus in grouper Epinephelus spp..ACTA VIROLOGICA,63(1),80-87.
MLA Yu, N-T,et al."A simplified method for the simultaneous detection of nervous necrosis virus and iridovirus in grouper Epinephelus spp.".ACTA VIROLOGICA 63.1(2019):80-87.

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来源：水生生物研究所

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