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Functional characterization of interleukin (IL)-22 and its inhibitor, IL-22 binding protein (IL-22BP) in Mandarin fish, Siniperca chuatsi

文献类型：期刊论文

作者Huo, Hui Jun1,2,3; Chen, Shan Nan1,2; Li, Li1,2; Laghari, Zubair Ahmed1,2; Li, Nan1,2; Nie, Pin1,2,4,5
刊名DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
出版日期2019-08-01
卷号97页码:88-97
关键词IL-22 IL-22BP Hepcidin LEAP-2 Mandarin fish
ISSN号0145-305X
DOI10.1016/j.dci.2019.03.007
通讯作者Nie, Pin(pinnie@ihb.ac.cn)
英文摘要As an important immune regulatory molecule, interleukin (IL)-22 has been reported in several species of fish, but its soluble receptor, IL 22 binding protein (IL-22BP), discovered as a natural antagonist of IL-22 in mammals, has not been functionally characterized in fish to date. In the present study, IL-22 and IL-22BP genes were cloned in mandarin fish Siniperca chuatsi. They all exhibited a high basal expression level in mucosa-enriched tissues, implying their possible roles in mucosal immunity. The IL-22 was found to show a potent response to LPS stimulation, acting as an inducer of antimicrobial peptide (AMP) genes, such as hepcidin and Liver-expressed antimicrobial peptide-2 (LEAP-2) in intestinal cells. IL-22BP, via co-incubation with IL-22, inhibited completely the induction of downstream genes by IL-22. Through a yeast two-hybrid assay, the interaction between IL-22BP and IL-22 was confirmed, which may account for the inhibitory effect of IL-22BP. Moreover, two hot spot residues for IL-22 binding, as reported in mammalian IL-22BP, were found to be conserved both in sequence location and function in mandarin fish IL-22BP, indicating that the interaction mode between IL-22 and IL-22BP may be also conserved in fish and mammals. In conclusion, the mandarin fish IL-22 and IL-22BP are conserved in their interaction and function with their mammalian orthologues, and these findings provide basis for future research on IL-22-IL-22BP axis in fish immunity.
WOS关键词T-CELL ; EXPRESSION ANALYSIS ; CRYSTAL-STRUCTURE ; INDUCIBLE FACTOR ; GENE-EXPRESSION ; IL-10 FAMILY ; IMMUNE CELLS ; HOST-DEFENSE ; IL-TIF ; CYTOKINE
资助项目National Natural Science Foundation of China[30230075] ; China Agriculture Research System[CARS-46] ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China
WOS研究方向Fisheries ; Immunology ; Zoology
语种英语
WOS记录号WOS:000468721600010
出版者ELSEVIER SCI LTD
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; China Agriculture Research System ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; special top talent plan One Thing One Decision (Yishiyiyi) in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China ; First class fishery discipline programme in Shandong Province, China
源URL[http://ir.ihb.ac.cn/handle/342005/28489]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者Nie, Pin
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
5.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China
推荐引用方式
GB/T 7714		 Huo, Hui Jun,Chen, Shan Nan,Li, Li,et al. Functional characterization of interleukin (IL)-22 and its inhibitor, IL-22 binding protein (IL-22BP) in Mandarin fish, Siniperca chuatsi[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2019,97:88-97.
APA Huo, Hui Jun,Chen, Shan Nan,Li, Li,Laghari, Zubair Ahmed,Li, Nan,&Nie, Pin.(2019).Functional characterization of interleukin (IL)-22 and its inhibitor, IL-22 binding protein (IL-22BP) in Mandarin fish, Siniperca chuatsi.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,97,88-97.
MLA Huo, Hui Jun,et al."Functional characterization of interleukin (IL)-22 and its inhibitor, IL-22 binding protein (IL-22BP) in Mandarin fish, Siniperca chuatsi".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 97(2019):88-97.

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来源：水生生物研究所

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