Identification, characterisation and preliminary functional analysis of IRAK-M in grass carp (Ctenopharyngodon idella)
文献类型：期刊论文
| 作者 | Chu, Pengfei1,2; He, Libo1; Zhu, Denghui1,2; Chen, Liangming1,2; Huang, Rong1; Liao, Lanjie1; Li, Yongming1; Zhu, Zuoyan1; Wang, Yaping1 |
| 刊名 | FISH & SHELLFISH IMMUNOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 84页码:312-321 |
| 关键词 | Grass carp IRAK-M Innate immune Subcellular localisation MyD88 |
| ISSN号 | 1050-4648 |
| DOI | 10.1016/j.fsi.2018.09.080 |
| 通讯作者 | Wang, Yaping(wangyp@ihb.ac.cn) |
| 英文摘要 | Interleukin-1 receptor-associated kinase (IRAK) family members play important roles in myeloid differentiation primary response 88 (MyD88)-dependent toll-like receptor (TLR) signaling, the crucial innate immune pathway in vertebrates. In the present study, the IRAK family gene IRAK-M (also called IRAK3) from grass carp (Ctenopharyngodon idella) was cloned and characterised. IRAK-M was mainly enriched in the spleen, and the significantly altered expression was observed after grass carp reovirus (GCRV) infection. Subcellular localisation showed that IRAK-M protein distributed uniformly in the entire cell and co-localised with MyD88 in the cytoplasm of transfected cells. Additionally, the interaction between IRAK-M and MyD88 was confirmed by bimolecular fluorescence complementation (BiFC) system. Moreover, deficient of IRAK-M in C. idella kidney cell line (CIK) with small interference RNA (siRNA) upregulated polyinosinic:polycytidylic acid (poly(I:C))-induced inflammatory cytokines production, including interleukin 8 (1L-8), 1L-6, and tumour necrosis factor alpha (TNF-alpha), which reveals that IRAK-M functions as a negative regulator of inflammatory cytokines. Taken together, our results demonstrate that IRAK-M gene plays an important role in innate immune regulation and provide new insights into understanding the functional characteristics of the IRAK-M in teleosts. |
| WOS关键词 | NF-KAPPA-B ; TOLL-LIKE RECEPTORS ; SIGNALING PATHWAYS ; GENE-EXPRESSION ; UP-REGULATION ; ACTIVATION ; AUTOPHOSPHORYLATION ; MACROPHAGES ; MOLECULES ; PROMOTES |
| 资助项目 | National Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31702332] ; Hubei Natural Science Foundation of China[2015CFA007] ; Hubei Natural Science Foundation of China[2017CFB374] ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences[XDA08030203] |
| WOS研究方向 | Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000457666800036 |
| 出版者 | ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Hubei Natural Science Foundation of China ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences ; Strategic Pilot Science and Technology Projects (A) Category, Chinese Academy of Sciences |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/28619]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 通讯作者 | Wang, Yaping |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chu, Pengfei,He, Libo,Zhu, Denghui,et al. Identification, characterisation and preliminary functional analysis of IRAK-M in grass carp (Ctenopharyngodon idella)[J]. FISH & SHELLFISH IMMUNOLOGY,2019,84:312-321. |
| APA | Chu, Pengfei.,He, Libo.,Zhu, Denghui.,Chen, Liangming.,Huang, Rong.,...&Wang, Yaping.(2019).Identification, characterisation and preliminary functional analysis of IRAK-M in grass carp (Ctenopharyngodon idella).FISH & SHELLFISH IMMUNOLOGY,84,312-321. |
| MLA | Chu, Pengfei,et al."Identification, characterisation and preliminary functional analysis of IRAK-M in grass carp (Ctenopharyngodon idella)".FISH & SHELLFISH IMMUNOLOGY 84(2019):312-321. |
入库方式： OAI收割
来源：水生生物研究所
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