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Isolation and characterization of microsatellite markers in a highland fish, Pareuchiloglanis sinensis (Siluriformes: Sisoridae) by next-generation sequencing

文献类型：期刊论文

作者Chen, Weitao1,2; He, Shunping1
刊名JOURNAL OF GENETICS
出版日期2018-12-01
卷号97页码:111-116
关键词microsatellite markers Dadu river RNA-seq Pareuchiloglanis sinensis
ISSN号0022-1333
DOI10.1007/s12041-018-0997-6
通讯作者He, Shunping(clad@ihb.ac.cn)
英文摘要Pareuchiloglanis sinensis (Siluriformes: Sisoridae) is an endemic and highland fish species which occurs only in some rivers of south-west China. In this study, the isolation and characterization of polymorphic microsatellite loci of this fish species by next-generation sequencing is described. A total of 9471 simple-sequence repeats (SSRs) were observed from RNA-seq data. One hundred and twenty primer pairs were chosen randomly and validated across 48 P. sinensis individuals collected from the Dadu river (a branch of the Yangtze river) of which 28 polymorphic microsatellite loci were detected. The number of alleles ranged from 2 to 14, with an average of seven alleles per locus. Twenty loci exhibited high polymorphism with the polymorphism information content (PIC) larger than 0.5. The mean observed and expected heterozygosity varied from 0.104 to 0.958 and 0.157 to 0.844, with an average of 0.583 and 0.613, respectively. The microsatellite markers characterized in the current study serve as a useful tool for the conversation genetic studies and population evaluation of P. sinensis.
资助项目National Natural Science Foundation of China[31130049] ; National Natural Science Foundation of China[U1036603]
WOS研究方向Genetics & Heredity
语种英语
WOS记录号WOS:000451438500014
出版者INDIAN ACAD SCIENCES
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://ir.ihb.ac.cn/handle/342005/28985]  
专题水生生物研究所_水生生物多样性与资源保护研究中心_期刊论文
通讯作者He, Shunping
作者单位1.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
2.CAFS, Pearl River Fisheries Res Inst, Guangzhou 510380, Guangdong, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Weitao,He, Shunping. Isolation and characterization of microsatellite markers in a highland fish, Pareuchiloglanis sinensis (Siluriformes: Sisoridae) by next-generation sequencing[J]. JOURNAL OF GENETICS,2018,97:111-116.
APA Chen, Weitao,&He, Shunping.(2018).Isolation and characterization of microsatellite markers in a highland fish, Pareuchiloglanis sinensis (Siluriformes: Sisoridae) by next-generation sequencing.JOURNAL OF GENETICS,97,111-116.
MLA Chen, Weitao,et al."Isolation and characterization of microsatellite markers in a highland fish, Pareuchiloglanis sinensis (Siluriformes: Sisoridae) by next-generation sequencing".JOURNAL OF GENETICS 97(2018):111-116.

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来源：水生生物研究所

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