Mitochondrial genomes of two diplectanids (Platyhelminthes: Monogenea) expose paraphyly of the order Dactylogyridea and extensive tRNA gene rearrangements
文献类型：期刊论文
| 作者 | Zhang, Dong1,2,3; Li, Wen X.1,2; Zou, Hong1,2; Wu, Shan G.1,2; Li, Ming1,2; Jakovlic, Ivan4; Zhang, Jin4; Chen, Rong4; Wang, Gui T.1,2 |
| 刊名 | PARASITES & VECTORS
 |
| 出版日期 | 2018-11-20 |
| 卷号 | 11页码:13 |
| 关键词 | Phylogenomics Gene rearrangement Molecular markers Gene loss Pseudo tRNA gene tandem array |
| ISSN号 | 1756-3305 |
| DOI | 10.1186/s13071-018-3144-6 |
| 通讯作者 | Wang, Gui T.(gtwang@ihb.ac.cn) |
| 英文摘要 | Background: Recent mitochondrial phylogenomics studies have reported a sister-group relationship of the orders Capsalidea and Dactylogyridea, which is inconsistent with previous morphology- and molecular-based phylogenies. As Dactylogyridea mitochondrial genomes (mitogenomes) are currently represented by only one family, to improve the phylogenetic resolution, we sequenced and characterized two dactylogyridean parasites, Lamellodiscus spari and Lepidotrema longipenis, belonging to a non-represented family Diplectanidae. Results: The L. longipenis mitogenome (15,433 bp) contains the standard 36 flatworm mitochondrial genes (atp8 is absent), whereas we failed to detect trnS1, trnC and trnG in L. spari (14,614 bp). Both mitogenomes exhibit unique gene orders (among the Monogenea), with a number of tRNA rearrangements. Both long non-coding regions contain a number of different (partially overlapping) repeat sequences. Intriguingly, these include putative tRNA pseudogenes in a tandem array (17 trnV pseudogenes in L. longipenis, 13 trnY pseudogenes in L. spari). Combined nucleotide diversity, non-synonymous/synonymous substitutions ratio and average sequence identity analyses consistently showed that nad2, nad5 and nad4 were the most variable PCGs, whereas cox1, cox2 and cytb were the most conserved. Phylogenomic analysis showed that the newly sequenced species of the family Diplectanidae formed a sister-group with the Dactylogyridae + Capsalidae clade. Thus Dactylogyridea (represented by the Diplectanidae and Dactylogyridae) was rendered paraphyletic (with high statistical support) by the nested Capsalidea (represented by the Capsalidae) clade. Conclusions: Our results show that nad2, nad5 and nad4 (fast-evolving) would be better candidates than cox1 (slow-evolving) for species identification and population genetics studies in the Diplectanidae. The unique gene order pattern further suggests discontinuous evolution of mitogenomic gene order arrangement in the Class Monogenea. This first report of paraphyly of the Dactylogyridea highlights the need to generate more molecular data for monogenean parasites, in order to be able to clarify their relationships using large datasets, as single-gene markers appear to provide a phylogenetic resolution which is too low for the task. |
| WOS关键词 | GYRODACTYLUS-SALARIS ; MOLECULAR PHYLOGENY ; PARASITIC FLATWORMS ; ATLANTIC SALMON ; DNA VARIATION ; SEQUENCE ; ANNOTATION ; EVOLUTION ; SOFTWARE ; TOOL |
| 资助项目 | National Natural Science Foundation of China[31872604] ; National Natural Science Foundation of China[31572658] ; Earmarked Fund for China Agriculture Research System[CARS-45-15] ; Major Scientific and Technological Innovation Project of Hubei Province[2015ABA045] |
| WOS研究方向 | Parasitology ; Tropical Medicine |
| 语种 | 英语 |
| WOS记录号 | WOS:000450847800002 |
| 出版者 | BMC |
| 资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Major Scientific and Technological Innovation Project of Hubei Province ; Major Scientific and Technological Innovation Project of Hubei Province ; Major Scientific and Technological Innovation Project of Hubei Province ; Major Scientific and Technological Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Major Scientific and Technological Innovation Project of Hubei Province ; Major Scientific and Technological Innovation Project of Hubei Province ; Major Scientific and Technological Innovation Project of Hubei Province ; Major Scientific and Technological Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Major Scientific and Technological Innovation Project of Hubei Province ; Major Scientific and Technological Innovation Project of Hubei Province ; Major Scientific and Technological Innovation Project of Hubei Province ; Major Scientific and Technological Innovation Project of Hubei Province ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Earmarked Fund for China Agriculture Research System ; Major Scientific and Technological Innovation Project of Hubei Province ; Major Scientific and Technological Innovation Project of Hubei Province ; Major Scientific and Technological Innovation Project of Hubei Province ; Major Scientific and Technological Innovation Project of Hubei Province |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/29083]  |
| 专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
| 通讯作者 | Wang, Gui T. |
| 作者单位 | 1.Chinese Acad Sci, Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Biotransduct Lab, Wuhan 430075, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Dong,Li, Wen X.,Zou, Hong,et al. Mitochondrial genomes of two diplectanids (Platyhelminthes: Monogenea) expose paraphyly of the order Dactylogyridea and extensive tRNA gene rearrangements[J]. PARASITES & VECTORS,2018,11:13. |
| APA | Zhang, Dong.,Li, Wen X..,Zou, Hong.,Wu, Shan G..,Li, Ming.,...&Wang, Gui T..(2018).Mitochondrial genomes of two diplectanids (Platyhelminthes: Monogenea) expose paraphyly of the order Dactylogyridea and extensive tRNA gene rearrangements.PARASITES & VECTORS,11,13. |
| MLA | Zhang, Dong,et al."Mitochondrial genomes of two diplectanids (Platyhelminthes: Monogenea) expose paraphyly of the order Dactylogyridea and extensive tRNA gene rearrangements".PARASITES & VECTORS 11(2018):13. |
入库方式： OAI收割
来源：水生生物研究所
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