Crystal-on-crystal chips for in situ serial diffraction at room temperature
文献类型：期刊论文
| 作者 | Ren, Zhong1,3; Ayhan, Medine1; Bandara, Sepatika1; Bowatte, Katinga1; Kumarapperuma, Indika1; Gunawardana, Semini1; Shin, Heewhan1; Wang, Cong1; Zeng, Xiaoti1,4; Yang, Xiaojing1,2 |
| 刊名 | LAB ON A CHIP
 |
| 出版日期 | 2018-08-07 |
| 卷号 | 18期号:15页码:2246-2256 |
| ISSN号 | 1473-0197 |
| DOI | 10.1039/c8lc00489g |
| 通讯作者 | Ren, Zhong(zren@uic.edu) ; Yang, Xiaojing(xiaojing@uic.edu) |
| 英文摘要 | Recent developments in serial crystallography at X-ray free electron lasers (XFELs) and synchrotrons have been driven by two scientific goals in structural biology - first, static structure determination from nano or microcrystals of membrane proteins and large complexes that are difficult for conventional cryocrystallography, and second, direct observations of transient structural species in biochemical reactions at near atomic resolution. Since room-temperature diffraction experiments naturally demand a large quantity of purified protein, sample economy is critically important for all steps of serial crystallography from crystallization, crystal delivery to data collection. Here we report the development and applications of "crystal-on-crystal" devices to facilitate large-scale in situ serial diffraction experiments on protein crystals of all sizes - large, small or microscopic. We show that the monocrystalline quartz as a substrate material prevents vapor loss during crystallization and significantly reduces background X-ray scattering. These devices can be readily adopted at XFEL and synchrotron beamlines, which enable efficient delivery of hundreds to millions of crystals to the X-ray beam, with an overall protein consumption per dataset comparable to that of cryocrystallography. |
| WOS关键词 | X-RAY-DIFFRACTION ; FREE-ELECTRON LASERS ; MACROMOLECULAR CRYSTALLOGRAPHY ; FEMTOSECOND CRYSTALLOGRAPHY ; PROTEIN CRYSTALLIZATION ; LAUE DIFFRACTION ; 6-4 PHOTOLYASE ; PLATFORM ; DEVICE ; RATES |
| 资助项目 | US Department of Energy[DE-AC02-06CH11357] ; National Institute of General Medical Sciences of the National Institutes of Health[R24GM111072] ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor[085P1000817] ; National Institutes of Health[R01EY024363] ; University of Illinois at Chicago |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry ; Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000448901700010 |
| 出版者 | ROYAL SOC CHEMISTRY |
| 资助机构 | US Department of Energy ; US Department of Energy ; US Department of Energy ; US Department of Energy ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; US Department of Energy ; US Department of Energy ; US Department of Energy ; US Department of Energy ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; US Department of Energy ; US Department of Energy ; US Department of Energy ; US Department of Energy ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; US Department of Energy ; US Department of Energy ; US Department of Energy ; US Department of Energy ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; National Institute of General Medical Sciences of the National Institutes of Health ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Economic Development Corporation ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; Michigan Technology Tri-Corridor ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; National Institutes of Health ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago ; University of Illinois at Chicago |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/29145]  |
| 专题 | 水生生物研究所_其他_期刊论文 |
| 通讯作者 | Ren, Zhong; Yang, Xiaojing |
| 作者单位 | 1.Univ Illinois, Dept Chem, Chicago, IL 60607 USA 2.Univ Illinois, Dept Ophthalmol & Vis Sci, Chicago, IL 60607 USA 3.Renz Res Inc, Westmont, IL 60559 USA 4.Chinese Acad Sci, Inst Hydrobiol, Wuhan, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ren, Zhong,Ayhan, Medine,Bandara, Sepatika,et al. Crystal-on-crystal chips for in situ serial diffraction at room temperature[J]. LAB ON A CHIP,2018,18(15):2246-2256. |
| APA | Ren, Zhong.,Ayhan, Medine.,Bandara, Sepatika.,Bowatte, Katinga.,Kumarapperuma, Indika.,...&Yang, Xiaojing.(2018).Crystal-on-crystal chips for in situ serial diffraction at room temperature.LAB ON A CHIP,18(15),2246-2256. |
| MLA | Ren, Zhong,et al."Crystal-on-crystal chips for in situ serial diffraction at room temperature".LAB ON A CHIP 18.15(2018):2246-2256. |
入库方式： OAI收割
来源：水生生物研究所
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