Deletion of the fih gene encoding an inhibitor of hypoxia-inducible factors increases hypoxia tolerance in zebrafish
文献类型：期刊论文
| 作者 | Cai, Xiaolian1,4; Zhang, Dawei1; Wang, Jing1; Liu, Xing1; Ouyang, Gang1; Xiao, Wuhan1,2,3,4 |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
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| 出版日期 | 2018-10-05 |
| 卷号 | 293期号:40页码:15370-15380 |
| 关键词 | hypoxia development hydroxylase zebrafish stress tolerance fih hif-1a hif-1b hif-2a hif-2b factor inhibiting HIF |
| ISSN号 | 0021-9258 |
| DOI | 10.1074/jbc.RA118.003004 |
| 通讯作者 | Xiao, Wuhan(w-xiao@ihb.ac.cn) |
| 英文摘要 | Many aerobic organisms have developed molecular mechanism to tolerate hypoxia, but the specifics of these mechanisms remain poorly understood. It is important to develop genetic methods that confer increased hypoxia tolerance to intensively farmed aquatic species, as these are maintained in environments with limited available oxygen. As an asparaginyl hydroxylase of hypoxia-inducible factors (HIFs), factor inhibiting HIF (FIH) inhibits transcriptional activation of hypoxia-inducible genes by blocking the association of HIFs with the transcriptional coactivators CREB-binding protein (CBP) and p300. Therefore, here we sought to test whether fih is involved in regulating hypoxia tolerance in the commonly used zebrafish model. Overexpressing the zebrafish fih gene in epithelioma papulosum cyprini (EPC) cells and embryos, we found that fih inhibits the transcriptional activation of zebrafish HIF- proteins. Using CRISPR/Cas9 to obtain fih-null zebrafish mutants, we noted that the fih deletion makes zebrafish more tolerant of hypoxic conditions than their WT siblings, but does not result in oxygen consumption rates that significantly differ from those of WT fish. Of note, we identified fewer apoptotic cells in adult fih-null zebrafish brains and in fih-null embryos, possibly explaining why the fih-null mutant had greater hypoxia tolerance than the WT. Moreover, the fih deletion up-regulated several hypoxia-inducible genes in fih-null zebrafish exposed to hypoxia. The findings of our study suggest that fih plays a role in hypoxia tolerance by affecting the rate of cellular apoptosis in zebrafish. |
| WOS关键词 | HIGH-ALTITUDE ADAPTATION ; FACTOR 3A FOXO3A ; TRANSCRIPTIONAL ACTIVITY ; SIGNALING PATHWAY ; INDUCED AUTOPHAGY ; CELL-DEATH ; BECLIN 1 ; HYDROXYLASE ; PHYSIOLOGY ; FISHES |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDA08010208] ; NSFC[31830101] ; NSFC[91631102] ; NSFC[31671315] ; NSFC[31721005] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000446549200004 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 资助机构 | Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; NSFC ; NSFC ; NSFC ; NSFC ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; NSFC ; NSFC ; NSFC ; NSFC ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; NSFC ; NSFC ; NSFC ; NSFC ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; NSFC ; NSFC ; NSFC ; NSFC |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/29170]  |
| 专题 | 水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文 |
| 通讯作者 | Xiao, Wuhan |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China 2.Chinese Acad Sci, Key Lab Aquat Biodivers & Conservat, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 3.Minist Agr, Key Lab Aquaculture Dis Control, Wuhan 430072, Hubei, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Cai, Xiaolian,Zhang, Dawei,Wang, Jing,et al. Deletion of the fih gene encoding an inhibitor of hypoxia-inducible factors increases hypoxia tolerance in zebrafish[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2018,293(40):15370-15380. |
| APA | Cai, Xiaolian,Zhang, Dawei,Wang, Jing,Liu, Xing,Ouyang, Gang,&Xiao, Wuhan.(2018).Deletion of the fih gene encoding an inhibitor of hypoxia-inducible factors increases hypoxia tolerance in zebrafish.JOURNAL OF BIOLOGICAL CHEMISTRY,293(40),15370-15380. |
| MLA | Cai, Xiaolian,et al."Deletion of the fih gene encoding an inhibitor of hypoxia-inducible factors increases hypoxia tolerance in zebrafish".JOURNAL OF BIOLOGICAL CHEMISTRY 293.40(2018):15370-15380. |
入库方式： OAI收割
来源：水生生物研究所
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