Numerous experimental simulations with different warming scenarios have been conducted to predict how algae will respond to warming, but their conclusions are sometimes contradictory to each other. This might be due to a failure to consider interspecific interactions. In this study, the dominant diatom species in a seasonal succession were isolated and verified to adapt to different temperature ranges by constant temperature experiment. Both unialgal and mixed cultures were exposed to two fluctuant temperature treatments that simulated the temperature variations from early spring to summer, with one treatment 4 degrees C higher (warming scenario) than the other. We found that the specific response of diatoms to warming was affected by interspecific interactions. Spring warming had no significant effect on eurythermal species and had a positive effect on the abundance of warm-adapted diatom species, but interspecific interactions reduced this promotional effect. Cold-adapted species had a negative response to spring warming in the presence of other diatom species but had a positive response to early spring warming in the absence of interspecific interactions. In addition, warming resulted in the growth of all diatom species peaking earlier in unialgal cultures, but this effect could be weakened or amplified by interspecies interactions in mixed cultures. Our results suggest that the specific diatom species with different optimal growth temperature ranges responding to warming were expected if there were no interspecific interactions. However, in natural environments, the inevitable and complex interspecific interactions will influence the responses of diatoms to warming. This important factor should not be ignored in the prediction of organism responses to climate warming.