Vicariance and/or long-distance dispersal shape the distribution patterns of many extant taxa, and orogenesis and/or climate fluctuations are key factors that drive the events of vicariance and/or dispersal. In this study, we yielded biogeographical inferences from Bayesian binary MCMC (BBM) analysis to explore the potential colonization histories of Opsariichthys bidens in China and to identify potential factors responsible for the colonization histories. Many vicariance and dispersal events were identified. The results suggested that O. bidens seemed to have originated from the Yangtze River and/or the Pearl River and experienced a Yangtze River-Pearl River split at 7.04 million years ago (Ma). BBM analysis revealed that the Pearl River populations had undergone expansions to the Hainan drainages, the Lancangjiang River (upper Mekong) and the Nanpanjiang River (upper branch of the Pearl River) at 0.74 Ma. Meanwhile, the Yangtze River populations expanded to the northeastern drainages during the Early Pleistocene. Subsequently, the northeastern drainages dispersed to the Yellow River during the Middle Pleistocene. More recently, the northeastern drainage populations also dispersed to the Huaihe River and back to the Yangtze River. A portion of the Pearl River populations originated from the Yangtze River dispersed to the Jiulongjiang River during the Middle Pleistocene. These time scales fit well with the uplift of the Qinghai-Tibet Plateau and Pleistocene glacial cycles, indicating the two factors played vital roles in shaping the colonization histories of O. bidens.