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Alternative splicing of GnRH2 and GnRH2-associated peptide plays roles in gonadal differentiation of the rice field eel, Monopterus albus

文献类型：期刊论文

作者Feng, Ke1,2; Luo, Hongrui1,2; Hou, Mingxi1,2; Li, Yongming1; Chen, Ji1; Zhu, Zuoyan1; Hu, Wei1
刊名GENERAL AND COMPARATIVE ENDOCRINOLOGY
出版日期2018-10-01
卷号267页码:9-17
关键词GnRH2 GnRH2-associated peptide Alternative splicing Gonadal differentiation Rice field eel
ISSN号0016-6480
DOI10.1016/j.ygcen.2018.05.021
通讯作者Hu, Wei(huwei@ihb.ac.cn)
英文摘要The rice field eel, Monopterus albus, is a protogynous hermaphrodite fish, in which the gonads are initially female ovaries which then transform into male testes. The exact mechanisms governing sex reversal in the rice field eel are unknown. In this study, a novel alternative splicing variant of GnRH2 (GnRH2-SV), retaining the second intron, was discovered in the gonad of the rice field eel. Compared to GnRH2, GnRH2-SV may give rise to a novel truncated GnRH2-associated peptide (New GAP2). The normal transcript of GnRH2 was primarily expressed in the brain, and could also be detected in the liver, spleen, ovary, and testis. However, GnRH2-SV was only expressed in the ovary and testis. During sex reversal, GnRH2 expression levels increased significantly at late stages; however, expression levels of GnRH2-SV were lower in ovary than in ovotestis and testis. We also examined the effect of three peptides (GnRHa, GAP2, and New GAP2) on gonadal sex differentiation during the third stage of ovarian development of the rice field eel. Compared to the control group, the expression of amh increased significantly following incubation with each of the three peptides. However, only New GAP2 stimulated the expression of sox9a1 mRNA in vitro. After intraperitoneal injection of GAP2, the expression of amh, foxl2, and cyp19a1a increased significantly after 12 h; the concentration of serum 11-KT was also significantly increased at the 12 h time point. Treatment with New GAP2 significantly increased the expression of amh, dmrt1a, and sox9a1, and also increased the concentration of serum 11-KT. After treated with GnRHa, the expression of amh, dmrt1a, sox9a1, cyp19a1a, and foxl2 increased significantly, as did the level of serum E2. These results indicated that both GAP2 and New GAP2 play a crucial role in inducing expression changes of sex-differentiation related genes, and may be involved in the gonadal development and sex reversal in the rice field eel.
WOS关键词GONADOTROPIN-RELEASING-HORMONE ; MESSENGER-RNA EXPRESSION ; SEABREAM SPARUS-AURATA ; SEX REVERSAL ; RICEFIELD EEL ; ACANTHOPAGRUS-SCHLEGELI ; CARASSIUS-AURATUS ; MORONE-SAXATILIS ; NEURONAL SYSTEMS ; ZEBRAFISH BRAIN
资助项目National Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31325026] ; National Natural Science Foundation of China[31461163006] ; Natural Science Foundation of Hubei[2015CFA007]
WOS研究方向Endocrinology & Metabolism
语种英语
WOS记录号WOS:000444366400002
出版者ACADEMIC PRESS INC ELSEVIER SCIENCE
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Hubei ; Natural Science Foundation of Hubei ; Natural Science Foundation of Hubei ; Natural Science Foundation of Hubei ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Hubei ; Natural Science Foundation of Hubei ; Natural Science Foundation of Hubei ; Natural Science Foundation of Hubei ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Hubei ; Natural Science Foundation of Hubei ; Natural Science Foundation of Hubei ; Natural Science Foundation of Hubei ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Natural Science Foundation of Hubei ; Natural Science Foundation of Hubei ; Natural Science Foundation of Hubei ; Natural Science Foundation of Hubei
源URL[http://ir.ihb.ac.cn/handle/342005/29339]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者Hu, Wei
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Feng, Ke,Luo, Hongrui,Hou, Mingxi,et al. Alternative splicing of GnRH2 and GnRH2-associated peptide plays roles in gonadal differentiation of the rice field eel, Monopterus albus[J]. GENERAL AND COMPARATIVE ENDOCRINOLOGY,2018,267:9-17.
APA Feng, Ke.,Luo, Hongrui.,Hou, Mingxi.,Li, Yongming.,Chen, Ji.,...&Hu, Wei.(2018).Alternative splicing of GnRH2 and GnRH2-associated peptide plays roles in gonadal differentiation of the rice field eel, Monopterus albus.GENERAL AND COMPARATIVE ENDOCRINOLOGY,267,9-17.
MLA Feng, Ke,et al."Alternative splicing of GnRH2 and GnRH2-associated peptide plays roles in gonadal differentiation of the rice field eel, Monopterus albus".GENERAL AND COMPARATIVE ENDOCRINOLOGY 267(2018):9-17.

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来源：水生生物研究所

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