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Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus

文献类型：期刊论文

作者Chen, Zhong-Yuan1; Li, Tao1; Gao, Xiao-Chan1; Wang, Chen-Fei2; Zhang, Qi-Ya1
刊名VIRUSES-BASEL
出版日期2018-02-01
卷号10期号:2页码:12
关键词ranavirus Andrias davidianus ranavirus Chinese giant salamander DNA vaccine protective immunity
ISSN号1999-4915
DOI10.3390/v10020052
通讯作者Zhang, Qi-Ya(zhangqy@ihb.ac.cn)
英文摘要Andrias davidianus ranavirus (ADRV) is an emerging viral pathogen that causes severe systemic hemorrhagic disease in Chinese giant salamanders. There is an urgent need for developing an effective vaccine against this fatal disease. In this study, DNA vaccines containing the ADRV 2L gene (pcDNA-2L) and the 58L gene (pcDNA-58L) were respectively constructed, and their immune protective effects were evaluated in Chinese giant salamanders. In vitro and in vivo expression of the vaccine plasmids were confirmed in transfected cells and muscle tissues of vaccinated Chinese giant salamanders by using immunoblot analysis or RT-PCR. Following ADRV challenge, the Chinese giant salamanders vaccinated with pcDNA-2L showed a relative percent survival (RPS) of 66.7%, which was significant higher than that in Chinese giant salamanders immunized with pcDNA-58L (RPS of 3.3%). Moreover, the specific antibody against ADRV was detected in Chinese giant salamanders vaccinated with pcDNA-2L at 14 and 21 days post-vaccination by indirect enzyme-linked immunosorbent assay (ELISA). Transcriptional analysis revealed that the expression levels of immune-related genes including type I interferon (IFN), myxovirus resistance (Mx), major histocompatibility complex class IA (MHCIA), and immunoglobulin M (IgM) were strongly up-regulated after vaccination with pcDNA-2L. Furthermore, vaccination with pcDNA-2L significantly suppressed the virus replication, which was seen by a low viral load in the spleen of Chinese giant salamander survivals after ADRV challenge. These results indicated that pcDNA-2L could induce a significant innate immune response and an adaptive immune response involving both humoral and cell-mediated immunity that conferred effective protection against ADRV infection, and might be a potential vaccine candidate for controlling ADRV disease in Chinese giant salamanders.
WOS关键词SINGAPORE GROUPER IRIDOVIRUS ; KIDNEY NECROSIS VIRUS ; ENVELOPE PROTEIN ; CELL-LINES ; ORF 53R ; FISH ; VACCINES ; IDENTIFICATION ; AMPHIBIANS ; XENOPUS
资助项目Strategic Priority Research Program of the Chinese Academy of Sciences[XDA08030202] ; National Natural Science Foundation of China[31430091] ; National Natural Science Foundation of China[31772890]
WOS研究方向Virology
语种英语
WOS记录号WOS:000427544100002
出版者MDPI
资助机构Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; Strategic Priority Research Program of the Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China
源URL[http://ir.ihb.ac.cn/handle/342005/29694]  
专题水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文
通讯作者Zhang, Qi-Ya
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan 430072, Peoples R China
2.Wangs Giant Salamander Breeding Profess Coopera, Shiyan 442013, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Zhong-Yuan,Li, Tao,Gao, Xiao-Chan,et al. Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus[J]. VIRUSES-BASEL,2018,10(2):12.
APA Chen, Zhong-Yuan,Li, Tao,Gao, Xiao-Chan,Wang, Chen-Fei,&Zhang, Qi-Ya.(2018).Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus.VIRUSES-BASEL,10(2),12.
MLA Chen, Zhong-Yuan,et al."Protective Immunity Induced by DNA Vaccination against Ranavirus Infection in Chinese Giant Salamander Andrias davidianus".VIRUSES-BASEL 10.2(2018):12.

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来源：水生生物研究所

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