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  5. 期刊论文
Excretion pattern and dynamics of glutathione detoxification of microcystins in Sprague Dawley rat

文献类型：期刊论文

作者Li, Wei1,2; He, Jun1; Chen, Jun1; Xie, Ping1
刊名CHEMOSPHERE
出版日期2018
卷号191期号:1页码:357-364
关键词Detoxification MCRR-GSH/Cys Excretion Rat
ISSN号0045-6535
DOI10.1016/j.chemosphere.2017.09.083
英文摘要

The excretion route and dynamics of the glutathione (GSH) conjugate of microcystin-RR (MCRR), MCRRGSH, were quantitatively studied in Sprague Dawley rat exposed with MCRR-GSH via liquid chromatography electrospray ionization mass spectrometry (LC-ESI-MS). In the MCRR-GSH-treated rat, the average MCRR-Cysteine (MCRR-Cys)/MCRR-GSH ratio reached as high as 105.3, which indicated that the intermediate conjugate MCRR-GSH was rapidly converted to the product compound MCRR-Cys. Besides, MCRR was consistently detected in MCRR-GSH-treated rat, which suggested that MCRR can be dissociated from the MCRR-GSH conjugate and the reversibility of the MC-GSH conjugate. Results of total MC contents analysis in excrement showed that the total MC contents in urine were significantly higher than those in feces. The ratio of the total MC content in urine to feces was as high as 129.3, which demonstrates that the urine is the main route of excretion after MCRR-GSH-treatment. In urine, the MCRR-Cys concentration was 27.8 -fold, 19.4-fold higher than MCRR-GSH and MCRR, respectively. Our results, for the first time, quantitatively found that MCRR-GSH was rapidly converted to MCRR-Cys after exposed to rat, and was excreted mainly through urine in the form of the MCRR-Cys conjugate. This study suggests that the GSH detoxification pathway of MCs could help to explain the greater sensitivity of mammals to MCs. (C) 2017 Elsevier Ltd. All rights reserved.

WOS关键词TANDEM MASS-SPECTROMETRY ; ANION TRANSPORTING POLYPEPTIDES ; PEPTIDE TOXIN ; HEPATOTOXIC MICROCYSTINS ; CYANOBACTERIAL TOXINS ; TISSUE DISTRIBUTION ; BIGHEAD CARP ; LR ; TOXICITY ; FISH
资助项目National Nature Science Foundations of China[31600423] ; Youth Foundations of Changchun University of Science and Technology[XQNJJ-2016-16]
WOS研究方向Environmental Sciences & Ecology
语种英语
WOS记录号WOS:000418208000041
出版者PERGAMON-ELSEVIER SCIENCE LTD
资助机构National Nature Science Foundations of China ; National Nature Science Foundations of China ; National Nature Science Foundations of China ; National Nature Science Foundations of China ; National Nature Science Foundations of China ; National Nature Science Foundations of China ; National Nature Science Foundations of China ; National Nature Science Foundations of China ; Youth Foundations of Changchun University of Science and Technology ; Youth Foundations of Changchun University of Science and Technology ; Youth Foundations of Changchun University of Science and Technology ; Youth Foundations of Changchun University of Science and Technology ; Youth Foundations of Changchun University of Science and Technology ; Youth Foundations of Changchun University of Science and Technology ; Youth Foundations of Changchun University of Science and Technology ; Youth Foundations of Changchun University of Science and Technology ; National Nature Science Foundations of China ; National Nature Science Foundations of China ; 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源URL[http://ir.ihb.ac.cn/handle/342005/30068]  
专题水生生物研究所_淡水生态学研究中心_期刊论文
通讯作者Chen, Jun; Xie, Ping
作者单位1.Univ Chinese Acad Sci, Donghu Expt Stn Lake Ecosyst, State Key Lab Freshwater Ecol & Biotechnol China, Inst Hydrobiol, Donghu South Rd 7, Wuhan 430072, Hubei, Peoples R China
2.Changchun Univ Sci & Technol, Sch Life Sci & Technol, Changchun 130022, Jilin, Peoples R China
推荐引用方式
GB/T 7714		 Li, Wei,He, Jun,Chen, Jun,et al. Excretion pattern and dynamics of glutathione detoxification of microcystins in Sprague Dawley rat[J]. CHEMOSPHERE,2018,191(1):357-364.
APA Li, Wei,He, Jun,Chen, Jun,&Xie, Ping.(2018).Excretion pattern and dynamics of glutathione detoxification of microcystins in Sprague Dawley rat.CHEMOSPHERE,191(1),357-364.
MLA Li, Wei,et al."Excretion pattern and dynamics of glutathione detoxification of microcystins in Sprague Dawley rat".CHEMOSPHERE 191.1(2018):357-364.

入库方式： OAI收割

来源：水生生物研究所

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