Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare
文献类型:期刊论文
作者 | Zhang Xiaolin1,2; Li Nan1; Qin Ting1; Huang Bei3; Nie Pin1 |
刊名 | CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY |
出版日期 | 2017-11-01 |
卷号 | 35期号:6页码:1511-1523 |
ISSN号 | 0254-4059 |
关键词 | Flavobacterium columnare GH19 domain gene deletion rhizoid colony non-rhizoid colony |
DOI | 10.1007/s00343-017-6160-z |
英文摘要 | Flavobacterium columnare is the pathogenic agent of columnaris disease in aquaculture. Using a recently developed gene deletion strategy, two genes that encode the Glyco_hydro_19 domain (GH19 domain) containing proteins, ghd-1 and ghd-2, were deleted separately and together from the F. columnare G(4) wild type strain. Surprisingly, the single-, Delta ghd-1 and Delta ghd-2, and double-gene mutants, Delta ghd-1 Delta ghd-2, all had rhizoid and non-rhizoid colony morphotypes, which we named Delta ghd-1, Delta ghd-2, Delta ghd-1 Delta ghd-2, and N Delta ghd-1, N Delta ghd-2, and N Delta ghd-1 Delta ghd-2. However, chitin utilization was not detected in either these mutants or in the wild type. Instead, skimmed milk degradation was observed for the mutants and the wild type; the non-rhizoid strain N Delta ghd-2 exhibited higher degradation activity as revealed by the larger transparent circle on the skimmed milk plate. Using zebrafish as the model organism, we found that non-rhizoid mutants had higher LD50 values and were less virulent because zebrafish infected with these survived longer. Transcriptome analysis between the non-rhizoid and rhizoid colony morphotypes of each mutant, i.e., N Delta ghd -1 versus (vs) Delta ghd-1, N Delta ghd-2 vs Delta ghd-2, and N Delta ghd-1 Delta ghd-2 vs Delta ghd-1 Delta ghd-2, revealed a large number of differentially expressed genes, among which 39 genes were common in three of the pairs compared. Although most of these genes encode hypothetical proteins, a few molecules such as phage tail protein, rhs element Vgr protein, thiol-activated cytolysin, and TonB-dependent outer membrane receptor precursor, expression of which was down-regulated in non-rhizoid mutants but up-regulated in rhizoid mutants, may play a role F. columnare virulence. |
WOS关键词 | CHONDROITIN AC LYASE ; IX SECRETION SYSTEM ; FISH PATHOGEN ; GLIDING MOTILITY ; FLEXIBACTER-COLUMNARIS ; GENOME SEQUENCE ; CHITIN ; ADHESION ; GENE ; PSYCHROPHILUM |
资助项目 | Chinese Academy of Sciences[XDA08010207] ; National Key Technology R& D Program of China[2012BAD25B02] ; State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ04] |
WOS研究方向 | Marine & Freshwater Biology ; Oceanography |
语种 | 英语 |
出版者 | SCIENCE PRESS |
WOS记录号 | WOS:000415810900022 |
资助机构 | Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; 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State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; 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National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; National Key Technology R& D Program of China ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; State Key Laboratory of Freshwater Ecology and Biotechnology ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; Chinese Academy of Sciences ; 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源URL | [http://ir.ihb.ac.cn/handle/342005/30182] |
专题 | 水生生物研究所_鱼类生物学及渔业生物技术研究中心_期刊论文 |
通讯作者 | Nie Pin |
作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Jimei Univ, Coll Fisheries, Xiamen 361021, Peoples R China |
推荐引用方式 GB/T 7714 | Zhang Xiaolin,Li Nan,Qin Ting,et al. Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare[J]. CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2017,35(6):1511-1523. |
APA | Zhang Xiaolin,Li Nan,Qin Ting,Huang Bei,&Nie Pin.(2017).Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare.CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY,35(6),1511-1523. |
MLA | Zhang Xiaolin,et al."Involvement of two glycoside hydrolase family 19 members in colony morphotype and virulence in Flavobacterium columnare".CHINESE JOURNAL OF OCEANOLOGY AND LIMNOLOGY 35.6(2017):1511-1523. |
入库方式: OAI收割
来源:水生生物研究所
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