Three Substrains of the Cyanobacterium Anabaena sp Strain PCC 7120 Display Divergence in Genomic Sequences and hetC Function
文献类型:期刊论文
作者 | Wang, Yali1,2; Gao, Yuan1; Gao, Hong1,2; Zhang, Cheng-Cai2; Xu, Xudong1,2 |
刊名 | JOURNAL OF BACTERIOLOGY |
出版日期 | 2018-07-01 |
卷号 | 200期号:13页码:13 |
ISSN号 | 0021-9193 |
关键词 | Anabaena sp strain PCC 7120 microevolution heterocyst differentiation |
DOI | 10.1128/JB.00076-18 |
英文摘要 | Anabaena sp. strain PCC 7120 is a model strain for molecular studies of cell differentiation and patterning in heterocyst-forming cyanobacteria. Subtle differences in heterocyst development have been noticed in different laboratories working on the same organism. In this study, 360 mutations, including single nucleotide polymorphisms (SNPs), small insertion/deletions (indels; 1 to 3 bp), fragment deletions, and transpositions, were identified in the genomes of three substrains. Heterogeneous/heterozygous bases were also identified due to the polyploidy nature of the genome and the multicellular morphology but could be completely segregated when plated after filament fragmentation by somcation. hetC is a gene upregulated in developing cells during heterocyst formation in Anabaena sp. strain PCC 7120 and found in approximately half of other heterocyst-forming cyanobacteria. Inactivation of hetC in 3 substrains of Anabaena sp. PCC 7120 led to different phenotypes: the formation of heterocysts, differentiating cells that keep dividing, or the presence of both heterocysts and dividing differentiating cells. The expression of P-het7-gfp in these hetC mutants also showed different patterns of green fluorescent protein (GFP) fluorescence. Thus, the function of hetC is influenced by the genomic background and epistasis and constitutes an example of evolution under way. IMPORTANCE Our knowledge about the molecular genetics of heterocyst formation, an important cell differentiation process for global N-2 fixation, is mostly based on studies with Anabaena sp. strain PCC 7120. Here, we show that rapid microevolution is under way in this strain, leading to phenotypic variations for certain genes related to heterocyst development, such as hetC. This study provides an example for ongoing microevolution, marked by multiple heterogeneous/heterozygous single nucleotide polymorphisms (SNPs), in a multicellular multicopy-genome microorganism. |
WOS关键词 | HETEROCYST PATTERN-FORMATION ; INTERCELLULAR TRANSFER ; INSERTION SEQUENCES ; RESPONSE REGULATOR ; ESCHERICHIA-COLI ; SP PCC-7120 ; DIFFERENTIATION ; GENE ; EXPRESSION ; EVOLUTION |
资助项目 | National Natural Science Foundation of China[31270132] ; National Natural Science Foundation of China[31770044] ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS[2016FBZ09] |
WOS研究方向 | Microbiology |
语种 | 英语 |
出版者 | AMER SOC MICROBIOLOGY |
WOS记录号 | WOS:000434807300004 |
资助机构 | National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS ; State Key Laboratory of Freshwater Ecology and Biotechnology at IHB, CAS |
源URL | [http://ir.ihb.ac.cn/handle/342005/30375] |
专题 | 水生生物研究所_藻类生物学及应用研究中心_期刊论文 |
通讯作者 | Xu, Xudong |
作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Key Lab Algal Biol, Wuhan, Hubei, Peoples R China |
推荐引用方式 GB/T 7714 | Wang, Yali,Gao, Yuan,Gao, Hong,et al. Three Substrains of the Cyanobacterium Anabaena sp Strain PCC 7120 Display Divergence in Genomic Sequences and hetC Function[J]. JOURNAL OF BACTERIOLOGY,2018,200(13):13. |
APA | Wang, Yali,Gao, Yuan,Gao, Hong,Zhang, Cheng-Cai,&Xu, Xudong.(2018).Three Substrains of the Cyanobacterium Anabaena sp Strain PCC 7120 Display Divergence in Genomic Sequences and hetC Function.JOURNAL OF BACTERIOLOGY,200(13),13. |
MLA | Wang, Yali,et al."Three Substrains of the Cyanobacterium Anabaena sp Strain PCC 7120 Display Divergence in Genomic Sequences and hetC Function".JOURNAL OF BACTERIOLOGY 200.13(2018):13. |
入库方式: OAI收割
来源:水生生物研究所
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