Geranylgeranylacetone induction of HSP90 alpha exerts cryoprotective effect on Acipenser sinensis sperm
文献类型：期刊论文
| 作者 | Xi, Meng Dan1,2,3; Li, Ping3,4,5; Du, Hao3,4; Qiao, Xin Mei3,4; Liu, Zhi Gang3,4; Wei, Wei Qi1,3,4 |
| 刊名 | ANIMAL REPRODUCTION SCIENCE
 |
| 出版日期 | 2018-06-01 |
| 卷号 | 193页码:19-25 |
| 关键词 | Cryopreservation Geranylgeranylacetone HSP90 alpha Sperm quality Sturgeon |
| ISSN号 | 0378-4320 |
| DOI | 10.1016/j.anireprosci.2018.03.027 |
| 通讯作者 | Wei, Wei Qi(weiqw@yfi.ac.cn) |
| 英文摘要 | Heat Shock Protein 90 (HSP90) is a fertility-associated protein, the expression of which positively correlates with sperm quality in many species. Geranylgeranylacetone (GGA) is reported to induce expression of HSP90. The present study aimed to investigate whether GGA induced expression of HSP90 in Acipenser sinensis sperm to exert a cryoprotective effect. Sperm from five male A. sinensis was combined with extender containing 20 mmol/L tris pH = 8.1, 10% v/v methanol, 2-5 mmol/L KC1, 15 mmol/L lactose, and 15 mmol/L trehalose, with GGA at 0, 14, 67, 135, 673, 1346, or 6731 mu mol/L. After cryopreservation and thawing, the percentage of motile spermatozoa, spermatozoon curvilinear velocity (VCL), straight-line velocity (VSL), average path velocity (VAP), acrosome integrity, and membrane integrity, as well as fertility were evaluated. Sperm quality increased with the increase of GGA to 673 mu mol/L, but decreased at higher concentrations. Expression levels of HSP90 alpha were detected by Western blot in sperm frozen with GGA at 673 mu mol/L (highest obtained sperm quality), 6731 mu mol/L (highest GGA concentration), and a control without GGA. The expression of HSP90 alpha increased with the increase in GGA, with lowest expression observed in the control. GGA was found to induce increase of HSP90 alpha, and this increase was associated with higher quality cryopreserved sperm at concentrations 5.673 mu mol/L. This research suggests a viable technique to increase the quality of cryopreserved A. sinensis sperm by adding GGA to induce expression of HSP90 alpha. |
| WOS关键词 | HEAT-SHOCK PROTEINS ; ATP-BINDING-SITE ; HUMAN-SPERMATOZOA ; GENE-EXPRESSION ; BULL SPERM ; 90 HSP90 ; IN-VIVO ; MOTILITY ; CRYOPRESERVATION ; STURGEON |
| 资助项目 | Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences[2015B02YQ01] ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences[2016GH04] ; National Natural Science Foundation of China[31402301] ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China[201203086] ; National Program on Key Basic Research Project (973 program)[2015CB150702] ; Ministry of Education, Youth and Sports of the Czech Republic[CZ.1.05/2.1.00/01.0024] ; Ministry of Education, Youth and Sports of the Czech Republic[L01205] ; Czech Science Foundation[16-02407Y] |
| WOS研究方向 | Agriculture ; Reproductive Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000433999000003 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; Special Scientific Research Fund for Central Non-profit Institutes, Chinese Academy of Fishery Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; Special Fund for Agro-scientific Research in the Public Interest of the Ministry of Agriculture of China ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; National Program on Key Basic Research Project (973 program) ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Ministry of Education, Youth and Sports of the Czech Republic ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation ; Czech Science Foundation |
| 源URL | [http://ir.ihb.ac.cn/handle/342005/30480]  |
| 专题 | 水生生物研究所_其他_期刊论文 |
| 通讯作者 | Wei, Wei Qi |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Key Lab Freshwater Biodivers Conservat, Minist Agr China, Wuhan 430223, Hubei, Peoples R China 4.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Sinoczech Joint Lab Fish Conservat & Biotechnol, Wuhan 430223, Hubei, Peoples R China 5.Univ South Bohemia Ceske Budejovice, Fac Fisheries & Protect Waters, South Bohemian Res Ctr Aquaculture & Biodivers Hy, Res Inst Fish Culture & Hydrobiol, Zatisi 728-2, Vodnany 38925, Czech Republic |
| 推荐引用方式 GB/T 7714 | Xi, Meng Dan,Li, Ping,Du, Hao,et al. Geranylgeranylacetone induction of HSP90 alpha exerts cryoprotective effect on Acipenser sinensis sperm[J]. ANIMAL REPRODUCTION SCIENCE,2018,193:19-25. |
| APA | Xi, Meng Dan,Li, Ping,Du, Hao,Qiao, Xin Mei,Liu, Zhi Gang,&Wei, Wei Qi.(2018).Geranylgeranylacetone induction of HSP90 alpha exerts cryoprotective effect on Acipenser sinensis sperm.ANIMAL REPRODUCTION SCIENCE,193,19-25. |
| MLA | Xi, Meng Dan,et al."Geranylgeranylacetone induction of HSP90 alpha exerts cryoprotective effect on Acipenser sinensis sperm".ANIMAL REPRODUCTION SCIENCE 193(2018):19-25. |
入库方式： OAI收割
来源：水生生物研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。