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  5. 期刊论文
Steroidogenic factor-1 hypermethylation in maternal rat blood could serve as a biomarker for intrauterine growth retardation

文献类型：期刊论文

作者Wu, Dong-Mei1; Ma, Liang-Peng1,3; Song, Gui-Li2; Long, Yong2; Liu, Han-Xiao1; Liu, Yang1; Ping, Jie1
刊名ONCOTARGET
出版日期2017-11-10
卷号8期号:56页码:96139-96153
ISSN号1949-2553
关键词steroidogenic factor-1 (SF-1) DNA methylation intrauterine growth retardation (IUGR) clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) biomarker
DOI10.18632/oncotarget.21767
英文摘要

Intrauterine growth retardation (IUGR) is a common obstetric complication lacking an optimal method for prenatal screening. DNA methylation profile in maternal blood holds significant promise for prenatal screening. Here, we aimed to screen out potential IUGR biomarkers in maternal blood from the perspective of DNA methylation. The IUGR rat model was established by prenatal maternal undernutrition. High-throughput bisulfite sequencing of genomic DNA methylation followed by functional clustering analysis for differentially methylated region (DMR)-associated genes demonstrated that genes regulating transcription had the most significantly changed DNA methylation status in maternal blood with IUGR. Genes about apoptosis and placental development were also changed. Besides increased placental apoptosis, IUGR rats demonstrated the same hypermethylated CpG sites of steroidogenic factor-1 (SF-1, a DMR-associated transcription factor about placenta) promoter in maternal blood and placentae. Further, ff1b, the SF-1 ortholog, was knocked out in zebrafish by CRISPR/Cas9 technology. The knock-out zebrafish demonstrated developmental inhibition and increased IUGR rates, which confirmed the role of SF-1 in IUGR development. Finally, hypermethylated SF-1 was observed in human maternal blood of IUGR. This study firstly presented distinct DNA methylation profile in maternal blood of IUGR and showed hypermethylated SF-1 could be a potential IUGR biomarker in maternal rat blood.

WOS关键词FETAL ADRENAL STEROIDOGENESIS ; DNA METHYLATION ; EPIGENETIC ALTERATIONS ; HISTONE ACETYLATION ; FOOD RESTRICTION ; OFFSPRING RATS ; EXPRESSION ; IUGR ; ASSOCIATION ; PREGNANCY
资助项目National Natural Science Foundation of China[81673215] ; National Natural Science Foundation of China[81273107] ; Applied Fundamental Research Project of Wuhan[2017060201010199]
WOS研究方向Oncology ; Cell Biology
语种英语
出版者IMPACT JOURNALS LLC
WOS记录号WOS:000414937900086
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan ; Applied Fundamental Research Project of Wuhan
源URL[http://ir.ihb.ac.cn/handle/342005/30809]  
专题水生生物研究所_水生生物分子与细胞生物学研究中心_期刊论文
通讯作者Ping, Jie
作者单位1.Wuhan Univ, Sch Basic Med Sci, Dept Pharmacol, Wuhan 430071, Hubei, Peoples R China
2.Chinese Acad Sci, Key Lab Biodivers & Conservat Aquat Organism, Inst Hydrobiol, Wuhan 430072, Hubei, Peoples R China
3.Wuhan First Hosp, Dept Pharm, Wuhan 430022, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Wu, Dong-Mei,Ma, Liang-Peng,Song, Gui-Li,et al. Steroidogenic factor-1 hypermethylation in maternal rat blood could serve as a biomarker for intrauterine growth retardation[J]. ONCOTARGET,2017,8(56):96139-96153.
APA Wu, Dong-Mei.,Ma, Liang-Peng.,Song, Gui-Li.,Long, Yong.,Liu, Han-Xiao.,...&Ping, Jie.(2017).Steroidogenic factor-1 hypermethylation in maternal rat blood could serve as a biomarker for intrauterine growth retardation.ONCOTARGET,8(56),96139-96153.
MLA Wu, Dong-Mei,et al."Steroidogenic factor-1 hypermethylation in maternal rat blood could serve as a biomarker for intrauterine growth retardation".ONCOTARGET 8.56(2017):96139-96153.

入库方式： OAI收割

来源：水生生物研究所

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