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Expression, Purification, and Characterization of Hemolytic Toxin from Virulent Aeromonas hydrophila

文献类型：期刊论文

作者Dong, Jing1,2; Ruan, Jing3; Xu, Ning1,2; Yang, Yibin1,2; Ai, Xiaohui1,2
刊名JOURNAL OF THE WORLD AQUACULTURE SOCIETY
出版日期2017-06-01
卷号48期号:3页码:531-536
ISSN号0893-8849
DOI10.1111/jwas.12351
英文摘要

Aeromonas hydrophila is a major pathogen in aquaculture in China; it is also an emerging pathogen in humans and animals. In recent years, several severe diseases, such as gastroenteritis, meningitis, and skin and soft tissue infections, have been found to be caused by A. hydrophila. Aerolysin and hemolysin are the most important virulence factors secreted by A. hydrophila. Therefore, new drugs targeted at these two toxins can be found in the therapy for A. hydrophila infections, reducing the usage of antibiotics. In this paper, we expressed and purified the aerolysin and hemolysin using a prokaryotic expression system. We found that aerolysin was more toxic than hemolysin, both in vitro and in vivo. The lethal dose (LD50) of aerolysin and hemolysin was 0.4 and 2.3 mu g per fish, respectively.

WOS关键词AEROLYSIN ; WATER ; PROAEROLYSIN ; GENES ; MODEL
资助项目National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS[2015JBFM37] ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS[2015JBFM29] ; Special Fund for Agro-Scientific Research in the Public Interest of China[201203085]
WOS研究方向Fisheries
语种英语
WOS记录号WOS:000402640300016
出版者WILEY
资助机构National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; 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Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; National Nonprofit Institute Research Grant of Freshwater Fisheries Research Center, CAFS ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China ; Special Fund for Agro-Scientific Research in the Public Interest of China
源URL[http://ir.ihb.ac.cn/handle/342005/31806]  
专题水生生物研究所_其他_期刊论文
通讯作者Dong, Jing; Ai, Xiaohui
作者单位1.Chinese Acad Fishery Sci, Yangtze River Fisheries Res Inst, Wuhan 430223, Peoples R China
2.Chinese Acad Fishery Sci, Freshwater Fisheries Res Ctr, Wuxi 214081, Peoples R China
3.Chinese Acad Sci, Inst Hydrobiol, Wuhan 430072, Peoples R China
推荐引用方式
GB/T 7714		 Dong, Jing,Ruan, Jing,Xu, Ning,et al. Expression, Purification, and Characterization of Hemolytic Toxin from Virulent Aeromonas hydrophila[J]. JOURNAL OF THE WORLD AQUACULTURE SOCIETY,2017,48(3):531-536.
APA Dong, Jing,Ruan, Jing,Xu, Ning,Yang, Yibin,&Ai, Xiaohui.(2017).Expression, Purification, and Characterization of Hemolytic Toxin from Virulent Aeromonas hydrophila.JOURNAL OF THE WORLD AQUACULTURE SOCIETY,48(3),531-536.
MLA Dong, Jing,et al."Expression, Purification, and Characterization of Hemolytic Toxin from Virulent Aeromonas hydrophila".JOURNAL OF THE WORLD AQUACULTURE SOCIETY 48.3(2017):531-536.

入库方式： OAI收割

来源：水生生物研究所

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