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Rapid discovery and functional characterization of diterpene synthases from basidiomycete fungi by genome mining

文献类型：期刊论文

作者Li, Xin-Lin1; Xu, Yu-Xing5; Li, Yuan3; Zhang, Rui1; Hu, Tian-Yuan1; Su, Ping3; Zhou, Man2; Tang, Ting2; Zeng, Ying4; Yang, Yan-Long2
刊名FUNGAL GENETICS AND BIOLOGY
出版日期2019-07-01
卷号128页码:36-42
ISSN号1087-1845
关键词Basidiomycete Ditepene synthase Genome mining Cembrane diterpene (-)-cyatha-3,12-diene Ent-kauran-16 alpha-ol
DOI10.1016/j.fgb.2019.03.007
通讯作者Yang, Yan-Long(ylyang@lzu.edu.cn) ; Gao, Wei(weigao@ccmu.edu.cn)
英文摘要Basidiomycete fungi are a rich source of bioactive diterpenoid secondary metabolites. However, compared with the large number of diterpene synthases (di-TPSs) identified in plants and ascomycete fungi, only three di-TPSs have been described from basidiomycete fungi. Large scale genome sequencing projects combined with the development of synthetic biology techniques now has enabled the rapidly discovery and characterization of di-TPSs from basidiomycete fungi. In this study, we discovered and functionally characterized four di-TPSs from 220 genome sequenced basidiomycete fungi by a combined strategy of genomic data mining, phylogenetic analysis and fast products characterization with synthetic biology techniques. Among them, SteTC1 of Stereum histurum was characterized as the first fungal cembrane diterpene synthase; PunTC of Punctularia strigosozonata and SerTC of Serpula lacryrnans were characterized as ent-kauran-16 alpha-ol synthase and DenTC3 of Dentipellis sp was characterized as a cyathane synthase. Our results provide opportunities for the discovery of new diterpenoids from basidiomycete fungi by genome mining.
WOS研究方向Genetics & Heredity ; Mycology
语种英语
WOS记录号WOS:000471736900005
源URL[http://ir.kib.ac.cn/handle/151853/67237]  
专题昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
昆明植物研究所_资源植物与生物技术所级重点实验室
通讯作者Yang, Yan-Long; Gao, Wei
作者单位1.Capital Med Univ, Sch Tradit Chinese Med, Beijing 100069, Peoples R China
2.Lanzhou Univ, Coll Chem & Chem Engn, State Key Lab Appl Organ Chem, Lanzhou 730000, Gansu, Peoples R China
3.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, Beijing 100700, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
5.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China
6.Capital Med Univ, Sch Pharmaceut Sci, Beijing 100069, Peoples R China
推荐引用方式
GB/T 7714		 Li, Xin-Lin,Xu, Yu-Xing,Li, Yuan,et al. Rapid discovery and functional characterization of diterpene synthases from basidiomycete fungi by genome mining[J]. FUNGAL GENETICS AND BIOLOGY,2019,128:36-42.
APA Li, Xin-Lin.,Xu, Yu-Xing.,Li, Yuan.,Zhang, Rui.,Hu, Tian-Yuan.,...&Gao, Wei.(2019).Rapid discovery and functional characterization of diterpene synthases from basidiomycete fungi by genome mining.FUNGAL GENETICS AND BIOLOGY,128,36-42.
MLA Li, Xin-Lin,et al."Rapid discovery and functional characterization of diterpene synthases from basidiomycete fungi by genome mining".FUNGAL GENETICS AND BIOLOGY 128(2019):36-42.

入库方式： OAI收割

来源：昆明植物研究所

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