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Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway

文献类型：期刊论文

作者Yang, Zhenyan1,2; Yang, Lifang1,4; Liu, Changkun1,2; Qin, Xujie3; Liu, Haiyang3; Chen, Jiahui1; Ji, Yunheng1,2
刊名FITOTERAPIA
出版日期2019-06-01
卷号135页码:52-63
关键词Steroidal saponins Biosynthesis Transcriptome Monocotyledons Cholesterol Cycloartenol pathway
ISSN号0367-326X
DOI10.1016/j.fitote.2019.04.008
通讯作者Chen, Jiahui(chenjh@mail.kib.ac.cn) ; Ji, Yunheng(jiyh@mail.kib.ac.cn)
英文摘要Steroidal saponins, one of the most diverse groups of plant-derived natural products, elicit biological and pharmacological activities; however, the genes involved in their biosynthesis and the corresponding biosynthetic pathway in monocotyledon plants remain unclear. This study aimed to identify genes involved in the biosynthesis of steroidal saponins by performing a comparative analysis among transcriptomes of Paris polyphylla var. chinensis (PPC), Ypsilandra thibetica (YT), and Polygonatum kingianum (PK). De novo transcriptome assemblies generated 57,537, 140,420, and 151,773 unigenes from PPC, YT, and PK, respectively, of which 56.54, 47.81, and 44.30% were successfully annotated, respectively. Among the transcriptomes for PPC, YT, and PK, we identified 194, 169, and 131; 17, 14, and 26; and, 80, 122, and 113 unigenes corresponding to terpenoid backbone biosynthesis; sesquiterpenoid and triterpenoid biosynthesis; and, steroid biosynthesis pathways, respectively. These genes are putatively involved in the biosynthesis of cholesterol that is the primary precursor of steroidal saponins. Phylogenetic analyses indicated that lanosterol synthase may be exclusive to dicotyledon plant species, and the cytochrome P450 unigenes were closely related to clusters CYP90B1 and CYP734A1, which are UDP-glycosyltransferases unigenes homologous with the UGT73 family. Thus, unigenes of beta-glucosidase may be candidate genes for catalysis of later period modifications of the steroidal saponin skeleton. Our data provide evidence to support the hypothesis that monocotyledons biosynthesize steroidal saponins from cholesterol via the cycloartenol pathway.
WOS研究方向Pharmacology & Pharmacy
语种英语
WOS记录号WOS:000470048000009
源URL[http://ir.kib.ac.cn/handle/151853/67405]  
专题昆明植物研究所_中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者Chen, Jiahui; Ji, Yunheng
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Integrat Conservat Plant Species E, Kunming 650201, Yunnan, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
4.Yunnan Univ, Sch Life Sci, Kunming 650201, Yunnan, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Zhenyan,Yang, Lifang,Liu, Changkun,et al. Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway[J]. FITOTERAPIA,2019,135:52-63.
APA Yang, Zhenyan.,Yang, Lifang.,Liu, Changkun.,Qin, Xujie.,Liu, Haiyang.,...&Ji, Yunheng.(2019).Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway.FITOTERAPIA,135,52-63.
MLA Yang, Zhenyan,et al."Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway".FITOTERAPIA 135(2019):52-63.

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来源：昆明植物研究所

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