Genome sequence of Malania oleifera, a tree with great value for nervonic acid production
文献类型：期刊论文
| 作者 | Xu, Chao-Qun1; Liu, Hui1; Zhou, Shan-Shan1; Zhang, Dong-Xu2; Zhao, Wei1; Wang, Sihai3; Chen, Fu4; Sun, Yan-Qiang1; Nie, Shuai1; Jia, Kai-Hua1 |
| 刊名 | GIGASCIENCE
 |
| 出版日期 | 2019-02-01 |
| 卷号 | 8期号:2页码:14 |
| 关键词 | de novo genome assembly vulnerable plant Malania nervonic acid transcriptomes |
| ISSN号 | 2047-217X |
| DOI | 10.1093/gigascience/giy164 |
| 通讯作者 | Ma, Yongpeng(mayongpeng@mail.kib.ac.cn) ; Mao, Jian-Feng(jianfeng.mao@bjfu.edu.cn) |
| 英文摘要 | Background Malania oleifera, a member of the Olacaceae family, is an IUCN red listed tree, endemic and restricted to the Karst region of southwest China. This tree's seed is valued for its high content of precious fatty acids (especially nervonic acid). However, studies on its genetic makeup and fatty acid biogenesis are severely hampered by a lack of molecular and genetic tools. Findings We generated 51 Gb and 135Gb of raw DNA sequences, using Pacific Biosciences (PacBio) single-molecule real-time and 10x Genomics sequencing, respectively. A final genome assembly, with a scaffold N50 size of 4.65 Mb and a total length of 1.51Gb, was obtained by primary assembly based on PacBio long reads plus scaffolding with 10x Genomics reads. Identified repeats constituted approximate to 82% of the genome, and 24,064 protein-coding genes were predicted with high support. The genome has low heterozygosity and shows no evidence for recent whole genome duplication. Metabolic pathway genes relating to the accumulation of long-chain fatty acid were identified and studied in detail. Conclusions Here, we provide the first genome assembly and gene annotation for M. oleifera. The availability of these resources will be of great importance for conservation biology and for the functional genomics of nervonic acid biosynthesis. |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000462551600008 |
| 源URL | [http://ir.kib.ac.cn/handle/151853/67692]  |
| 专题 | 昆明植物研究所_资源植物与生物技术所级重点实验室 |
| 通讯作者 | Ma, Yongpeng; Mao, Jian-Feng |
| 作者单位 | 1.Beijing Forestry Univ, Sch Nat Conservat, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Natl Engn Lab Tree Breeding,Coll Biol Sci & Techn, Beijing 100083, Peoples R China 2.Datong Univ, Coll Life Sci, Datong 037009, Shanxi, Peoples R China 3.Yunnan Acad Forestry, State Forestry Adm Key Lab Yunnan Rare & Endanger, Yunnan Key Lab Forest Plant Cultivat & Utilizat, Kunming 650201, Yunnan, Peoples R China 4.Yunnan Acad Forestry, Camellia Inst, Guangnan 663300, Yunnan, Peoples R China 5.Beijing Ori Gene Sci & Technol Co Ltd, Beijing 102206, Peoples R China 6.Univ Georgia, Dept Genet, Athens, GA 30602 USA 7.Univ Nat Resources & Life Sci, PBU, Dept Biotechnol, BOKU VIBT, Muthgasse 18, A-1190 Vienna, Austria 8.Univ Laval, Dept Sci Bois & Foret, 1030 Ave Med, Quebec City, PQ G1V 0A6, Canada 9.Univ Laval, Inst Syst & Integrated Biol, Pavillon Charles Eugene Marchand,1030 Ave Med, Quebec City, PQ G1V 0A6, Canada 10.Univ Laval, Ctr Etud Foret, 1030 Ave Med, Quebec City, PQ G1V 0A6, Canada |
| 推荐引用方式 GB/T 7714 | Xu, Chao-Qun,Liu, Hui,Zhou, Shan-Shan,et al. Genome sequence of Malania oleifera, a tree with great value for nervonic acid production[J]. GIGASCIENCE,2019,8(2):14. |
| APA | Xu, Chao-Qun.,Liu, Hui.,Zhou, Shan-Shan.,Zhang, Dong-Xu.,Zhao, Wei.,...&Mao, Jian-Feng.(2019).Genome sequence of Malania oleifera, a tree with great value for nervonic acid production.GIGASCIENCE,8(2),14. |
| MLA | Xu, Chao-Qun,et al."Genome sequence of Malania oleifera, a tree with great value for nervonic acid production".GIGASCIENCE 8.2(2019):14. |
入库方式： OAI收割
来源：昆明植物研究所
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