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Recombinant polyphenol oxidases for production of theaflavins from tea polyphenols

文献类型：期刊论文

作者Zeng, Jun1,2; Du, Gang1; Shao, Xue1,2; Feng, Ke-Na1,2; Zeng, Ying1
刊名INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
出版日期2019-08-01
卷号134页码:139-145
ISSN号0141-8130
关键词Theaflavins Recombinant polyphenol oxidase Mesoporous silica
DOI10.1016/j.ijbiomac.2019.04.142
通讯作者Zeng, Ying(biochem@mail.kib.ac.cn)
英文摘要Theaflavins (TFs) have attracted much attention due to their various bioactivities in black tea. This paper describes the first trial for enzymatic production of TFs by recombinant polyphenol oxidases (PPOs). PPO genes were cloned from nine species and expressed in E. coli. Crude enzyme assays by LC-MS revealed that eight recombinant PPOs were active for TFs production from tea polyphenols as substrates. Much higher activities were observed for crude enzymes of Md2 from Malus domestica (apple), Pp4 from Pyrus pashia (pear), and Ej2 from Eriobotrya japonica (loquat). When immobilized on mesoporous silica, crude Md2 was most active. The purified Md2 was immobilized and showed almost twice activity as high as its free enzyme. While the maximum activity of free enzyme was found at pH 5 and 10-30 degrees C, the immobilized enzyme had broader range of pH 4-6 and 10-40 degrees C. The activity of immobilized enzyme was relatively constant during the pH and thermal stability test. When used at 0.2 mg/ml in the beginning, the immobilized enzyme retained approximately 40% of its initial activity after 8 cycles of operation. (C) 2019 Elsevier B.V. All rights reserved.
WOS研究方向Biochemistry & Molecular Biology ; Chemistry ; Polymer Science
语种英语
WOS记录号WOS:000474673600016
源URL[http://ir.kib.ac.cn/handle/151853/67840]  
专题昆明植物研究所_植物化学与西部植物资源持续利用国家重点实验室
通讯作者Zeng, Ying
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Nat Med Chem, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Zeng, Jun,Du, Gang,Shao, Xue,et al. Recombinant polyphenol oxidases for production of theaflavins from tea polyphenols[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,134:139-145.
APA Zeng, Jun,Du, Gang,Shao, Xue,Feng, Ke-Na,&Zeng, Ying.(2019).Recombinant polyphenol oxidases for production of theaflavins from tea polyphenols.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,134,139-145.
MLA Zeng, Jun,et al."Recombinant polyphenol oxidases for production of theaflavins from tea polyphenols".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 134(2019):139-145.

入库方式： OAI收割

来源：昆明植物研究所

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