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Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus

文献类型：期刊论文

作者Xu, Fuqiang1,2,3,4; Jin, Sen2; Liu, Qing3,4; Hu, Liang3; Lv, Pei3; Zhu, Xutao2,3; Jia, Fan2,3,4
刊名NEUROSCIENCE BULLETIN
出版日期2019-06-01
卷号35期号:3页码:378-388
关键词Semliki Forest virus Mutant virus-like particle Rapid labeling Sparse labeling Neuronal morphology
ISSN号1673-7067
DOI10.1007/s12264-019-00362-z
英文摘要Sparse labeling of neurons contributes to uncovering their morphology, and rapid expression of a fluorescent protein reduces the experiment range. To achieve the goal of rapid and sparse labeling of neurons in vivo, we established a rapid method for depicting the fine structure of neurons at 24 h post-infection based on a mutant virus-like particle of Semliki Forest virus. Approximately 0.014 fluorescent focus-forming units of the mutant virus-like particle transferred enhanced green fluorescent protein into neurons in vivo, and its affinity for neurons in vivo was stronger than for neurons in vitro and BHK21 (baby hamster kidney) cells. Collectively, the mutant virus-like particle provides a robust and convenient way to reveal the fine structure of neurons and is expected to be a helper virus for combining with other tools to determine their connectivity. Our work adds a new tool to the approaches for rapid and sparse labeling of neurons in vivo.
WOS关键词SINDBIS VIRUS ; MOSSY CELLS ; ADENOASSOCIATED VIRUS ; GENE-EXPRESSION ; PURKINJE-CELLS ; GRANULE CELLS ; VECTORS ; SYSTEM ; ALPHAVIRUSES ; ADENOVIRUS
资助项目National Natural Science Foundation of China[31771197] ; National Natural Science Foundation of China[31830035] ; National Natural Science Foundation of China[91732304] ; National Basic Research Development Program (973 Program) of China[2015CB755600] ; Strategic Priority Research Program (B), Chinese Academy of Sciences, China[XDBS01030200] ; Major Research Plan of the National Natural Science Foundation of China[91632303]
WOS研究方向Neurosciences & Neurology
语种英语
WOS记录号WOS:000468473500002
出版者SPRINGER
资助机构National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Development Program (973 Program) of China ; National Basic Research Development Program (973 Program) of China ; Strategic Priority Research Program (B), Chinese Academy of Sciences, China ; Strategic Priority Research Program (B), Chinese Academy of Sciences, China ; Major Research Plan of the National Natural Science Foundation of China ; Major Research Plan of the National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Development Program (973 Program) of China ; National Basic Research Development Program (973 Program) of China ; Strategic Priority Research Program (B), Chinese Academy of Sciences, China ; Strategic Priority Research Program (B), Chinese Academy of Sciences, China ; Major Research Plan of the National Natural Science Foundation of China ; Major Research Plan of the National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Development Program (973 Program) of China ; National Basic Research Development Program (973 Program) of China ; Strategic Priority Research Program (B), Chinese Academy of Sciences, China ; Strategic Priority Research Program (B), Chinese Academy of Sciences, China ; Major Research Plan of the National Natural Science Foundation of China ; Major Research Plan of the National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Basic Research Development Program (973 Program) of China ; National Basic Research Development Program (973 Program) of China ; Strategic Priority Research Program (B), Chinese Academy of Sciences, China ; Strategic Priority Research Program (B), Chinese Academy of Sciences, China ; Major Research Plan of the National Natural Science Foundation of China ; Major Research Plan of the National Natural Science Foundation of China
源URL[http://ir.wipm.ac.cn/handle/112942/13700]  
专题中国科学院武汉物理与数学研究所
通讯作者Xu, Fuqiang; Jia, Fan
作者单位1.Chinese Acad Sci, Ctr Excellence Brain Sci & Intelligence Technol, Shanghai 200031, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Phys & Math, Brain Res Ctr, Wuhan 430071, Hubei, Peoples R China
4.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom & Mol Phys, Key Lab Magnet Resonance Biol Syst, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Xu, Fuqiang,Jin, Sen,Liu, Qing,et al. Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus[J]. NEUROSCIENCE BULLETIN,2019,35(3):378-388.
APA Xu, Fuqiang.,Jin, Sen.,Liu, Qing.,Hu, Liang.,Lv, Pei.,...&Jia, Fan.(2019).Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus.NEUROSCIENCE BULLETIN,35(3),378-388.
MLA Xu, Fuqiang,et al."Rapid and Sparse Labeling of Neurons Based on the Mutant Virus-Like Particle of Semliki Forest Virus".NEUROSCIENCE BULLETIN 35.3(2019):378-388.

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来源：武汉物理与数学研究所

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