Chemical shift assignments of a camelid nanobody against aflatoxin B-1
文献类型：期刊论文
| 作者 | Yang, Yunhuang2; He, Ting2; Liu, Maili2; Hu, Rui2; Zhu, Jiang2; Li, Shuangli1,2; Nie, Yao1,2 |
| 刊名 | BIOMOLECULAR NMR ASSIGNMENTS
 |
| 出版日期 | 2019-04-01 |
| 卷号 | 13期号:1页码:75-78 |
| ISSN号 | 1874-2718 |
| 关键词 | Camelid antibody Nanobody Aflatoxin B-1 NMR spectroscopy Assignment |
| DOI | 10.1007/s12104-018-9855-y |
| 英文摘要 | Nanobodies (Nbs) are the variable domain of the heavy-chain antibodies produced from Camelidae, which possess comparable binding affinities and specificity to conventional antibodies. Nbs have become valuable and versatile tools for numerous biotechnology applications due to their small size (12-15kDa), high solubility, exceptional stability, and facile genetic manipulation. The interactions between Nbs and protein antigens have been well-studied, but less work has been done to characterize their ability to bind small molecule haptens. Here we present the backbone and side-chain assignments of the H-1, C-13 and N-15 resonances of Nb26 (123 amino acids), a nanobody that recognizes the hapten aflatoxin B-1 (AFB(1)). These assignments are preliminary work towards the determination of the structure of free Nb26 using NMR spectroscopy, which will provide useful information about the complex structure of Nb26-AFB(1) and the recognition mechanism about how Nb26 binds to AFB(1). |
| 资助项目 | "Hundred Talents Program" of Chinese Academy of Sciences ; National Natural Science Foundation of China[21575155] |
| WOS研究方向 | Biophysics ; Spectroscopy |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000463649500016 |
| 资助机构 | "Hundred Talents Program" of Chinese Academy of Sciences ; "Hundred Talents Program" of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; "Hundred Talents Program" of Chinese Academy of Sciences ; "Hundred Talents Program" of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; "Hundred Talents Program" of Chinese Academy of Sciences ; "Hundred Talents Program" of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; "Hundred Talents Program" of Chinese Academy of Sciences ; "Hundred Talents Program" of Chinese Academy of Sciences ; National Natural Science Foundation of China ; National Natural Science Foundation of China |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/13776]  |
| 专题 | 中国科学院武汉物理与数学研究所 |
| 通讯作者 | Yang, Yunhuang; He, Ting |
| 作者单位 | 1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan Ctr Magnet Resonance, State Key Lab Magnet Resonance & Atom Mol Phys, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Yunhuang,He, Ting,Liu, Maili,et al. Chemical shift assignments of a camelid nanobody against aflatoxin B-1[J]. BIOMOLECULAR NMR ASSIGNMENTS,2019,13(1):75-78. |
| APA | Yang, Yunhuang.,He, Ting.,Liu, Maili.,Hu, Rui.,Zhu, Jiang.,...&Nie, Yao.(2019).Chemical shift assignments of a camelid nanobody against aflatoxin B-1.BIOMOLECULAR NMR ASSIGNMENTS,13(1),75-78. |
| MLA | Yang, Yunhuang,et al."Chemical shift assignments of a camelid nanobody against aflatoxin B-1".BIOMOLECULAR NMR ASSIGNMENTS 13.1(2019):75-78. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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