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Uncorrelated Effect of Interdomain Contact on Pin1 Isomerase Activity Reveals Positive Catalytic Cooperativity

文献类型：期刊论文

作者Yang, Yunhuang2; Zhu, Jiang2; Liu, Maili1,2; Li, Ying2; Zhu, Wenkai1,2
刊名JOURNAL OF PHYSICAL CHEMISTRY LETTERS
出版日期2019-03-21
卷号10期号:6页码:1272-1278
ISSN号1948-7185
DOI10.1021/acs.jpclett.9b00052
英文摘要Pin1 is a two-domain peptidyl-prolyl isomerase (PPIase) associated with neurodegeneration and tumorigenesis. The two domains, a WW and a PPIase domain, are connected by a flexible linker, making Pin1 adopt various conformations ranging from compact to extended, wherein Pin1 exhibits different extents of interdomain contact. Previous studies have shown that weakening interdomain contact increases the isomerase activity of Pin1. Here, we propose an NMR chemical shift correlation-analysis-based method that will be general for two-domain proteins to gauge two-state populations of Pin1, and we report a linker-modified mutant of Pin1 with enhanced interdomain contact and increased isomerase activity, with the latter suggesting an uncorrelated effect of interdomain contact on isomerase activity. Thus, although bindings of different substrates in the WW domain impose opposite effects on interdomain contact, in both cases, it may promote isomerization, implying cooperativity between substrate binding in the WW domain and isomerization in the PPIase domain.
WOS关键词SUBSTRATE RECOGNITION ; NEGATIVE REGULATION ; STRUCTURAL BASIS ; PROLYL ; ISOMERIZATION ; PATHWAYS
资助项目National Key R&D Program of China[2016YFA051201] ; National Natural Sciences Foundation of China[21575155] ; National Natural Sciences Foundation of China[21735007] ; National Natural Sciences Foundation of China[21703283] ; K. C. Wong Educational Foundation ; Hundred Talent Program by Chinese Academy of Sciences
WOS研究方向Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics
语种英语
出版者AMER CHEMICAL SOC
WOS记录号WOS:000463678800017
资助机构National Key R&D Program of China ; National Key R&D Program of China ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; K. C. Wong Educational Foundation ; K. C. Wong Educational Foundation ; Hundred Talent Program by Chinese Academy of Sciences ; Hundred Talent Program by Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; K. C. Wong Educational Foundation ; K. C. Wong Educational Foundation ; Hundred Talent Program by Chinese Academy of Sciences ; Hundred Talent Program by Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; K. C. Wong Educational Foundation ; K. C. Wong Educational Foundation ; Hundred Talent Program by Chinese Academy of Sciences ; Hundred Talent Program by Chinese Academy of Sciences ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Sciences Foundation of China ; National Natural Sciences Foundation of China ; K. C. Wong Educational Foundation ; K. C. Wong Educational Foundation ; Hundred Talent Program by Chinese Academy of Sciences ; Hundred Talent Program by Chinese Academy of Sciences
源URL[http://ir.wipm.ac.cn/handle/112942/13792]  
专题中国科学院武汉物理与数学研究所
通讯作者Zhu, Jiang; Liu, Maili
作者单位1.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
2.Chinese Acad Sci, State Key Lab Magnet Resonance & Atom Mol Phys, Key Lab Magnet Resonance Biol Syst, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Peoples R China
推荐引用方式
GB/T 7714		 Yang, Yunhuang,Zhu, Jiang,Liu, Maili,et al. Uncorrelated Effect of Interdomain Contact on Pin1 Isomerase Activity Reveals Positive Catalytic Cooperativity[J]. JOURNAL OF PHYSICAL CHEMISTRY LETTERS,2019,10(6):1272-1278.
APA Yang, Yunhuang,Zhu, Jiang,Liu, Maili,Li, Ying,&Zhu, Wenkai.(2019).Uncorrelated Effect of Interdomain Contact on Pin1 Isomerase Activity Reveals Positive Catalytic Cooperativity.JOURNAL OF PHYSICAL CHEMISTRY LETTERS,10(6),1272-1278.
MLA Yang, Yunhuang,et al."Uncorrelated Effect of Interdomain Contact on Pin1 Isomerase Activity Reveals Positive Catalytic Cooperativity".JOURNAL OF PHYSICAL CHEMISTRY LETTERS 10.6(2019):1272-1278.

入库方式： OAI收割

来源：武汉物理与数学研究所

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