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An Aggregation-induced Emission Probe Based on Host-Guest Inclusion Composed of the Tetraphenylethylene Motif and gamma-Cyclodextrin for the Detection of alpha-Amylase

文献类型：期刊论文

作者Tang, Bo2; Li, Lu2; Shangguan, Huijuan3; Wan, Chuyun3; Chai, Zhaofei1; Li, Ya3; Deng, Qianchun3; Shi, Jie2,3
刊名CHEMISTRY-AN ASIAN JOURNAL
出版日期2019-03-15
卷号14期号:6页码:847-852
关键词aggregation-induced emission fluorescence fluorescent probes host-guest systems alpha-amylase
ISSN号1861-4728
DOI10.1002/asia.201801601
英文摘要alpha-Amylase, an essential biomarker in pancreas related diseases and perform a major role in carbohydrates metabolism. Hence, monitoring the dynamic changes of alpha-amylase is crucial for better clinical diagnosis of diseases. However, the existing methods are suffered from low sensitivity, time consumption and indirect assay with aid of tool enzyme or inhibitor of competitive substrates, the rapid and non-destructive sensing of alpha-amylase in biological samples was highly desired. In this work, a very simple tetraphenylethylene motif and gamma-cyclodextrin based supramolecular fluometric sensing system was firstly established. This system has no emission signal in aqueous media for the freely rotation of phenyl rings in the cavity of gamma-cyclodextrin, but the AIE residues can be released in to water after the alpha-amylase hydrolysing gamma-cyclodextrin, then turn on the fluorescence. In this system, the detection limit is calculated to be 0.007 U mL(-1) in MES buffer with a linear range of 0-0.35 U mL(-1), having excellent selectivity to alpha-amylase compared to other proteins. At last, our probe can be applied to the quantitative analysis of alpha-amylase in human serum, showing potential in point of care testing.
WOS关键词2-PHOTON FLUORESCENT-PROBE ; LIPASE ; DIAGNOSIS ; SERUM ; PANCREATITIS ; AIEGEN ; SENSE ; ASSAY
资助项目National Science Foundation of China[21535004] ; National Science Foundation of China[91753111] ; National Science Foundation of China[31872896] ; National Science Foundation of China[31501423] ; National Science Foundation of China[31501409] ; National Science Foundation of China[21390411] ; Young Elite Scientists Sponsorship Program by CAST[2017QNRC001] ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences[CAAS-ASTIP-2013-OCRI]
WOS研究方向Chemistry
语种英语
WOS记录号WOS:000461836500022
出版者WILEY-V C H VERLAG GMBH
资助机构National Science Foundation of China ; National Science Foundation of China ; Young Elite Scientists Sponsorship Program by CAST ; Young Elite Scientists Sponsorship Program by CAST ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; National Science Foundation of China ; National Science Foundation of China ; Young Elite Scientists Sponsorship Program by CAST ; Young Elite Scientists Sponsorship Program by CAST ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; National Science Foundation of China ; National Science Foundation of China ; Young Elite Scientists Sponsorship Program by CAST ; Young Elite Scientists Sponsorship Program by CAST ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; National Science Foundation of China ; National Science Foundation of China ; Young Elite Scientists Sponsorship Program by CAST ; Young Elite Scientists Sponsorship Program by CAST ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences ; Agricultural Science and Technology Inno-vation Project of Chinese Academy of Agricultural Sciences
源URL[http://ir.wipm.ac.cn/handle/112942/13852]  
专题中国科学院武汉物理与数学研究所
通讯作者Tang, Bo
作者单位1.Chinese Acad Sci, Wuhan Inst Phys & Math, State Key Lab Magnet Resonance & Atom & Mol Phys, Wuhan 430071, Hubei, Peoples R China
2.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Minist Educ, Key Lab Mol & Nano Probes, Jinan 250014, Shandong, Peoples R China
3.Chinese Acad Agr Sci, Oil Crops Res Inst, Hubei Key Lab Lipid Chem & Nutr, Key Lab Oilseeds Proc,Minist Agr, Wuhan 430062, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Tang, Bo,Li, Lu,Shangguan, Huijuan,et al. An Aggregation-induced Emission Probe Based on Host-Guest Inclusion Composed of the Tetraphenylethylene Motif and gamma-Cyclodextrin for the Detection of alpha-Amylase[J]. CHEMISTRY-AN ASIAN JOURNAL,2019,14(6):847-852.
APA Tang, Bo.,Li, Lu.,Shangguan, Huijuan.,Wan, Chuyun.,Chai, Zhaofei.,...&Shi, Jie.(2019).An Aggregation-induced Emission Probe Based on Host-Guest Inclusion Composed of the Tetraphenylethylene Motif and gamma-Cyclodextrin for the Detection of alpha-Amylase.CHEMISTRY-AN ASIAN JOURNAL,14(6),847-852.
MLA Tang, Bo,et al."An Aggregation-induced Emission Probe Based on Host-Guest Inclusion Composed of the Tetraphenylethylene Motif and gamma-Cyclodextrin for the Detection of alpha-Amylase".CHEMISTRY-AN ASIAN JOURNAL 14.6(2019):847-852.

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来源：武汉物理与数学研究所

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