中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 武汉物理与数学研究所
  3. 中国科学院武汉物理与数学研究所
Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication

文献类型：期刊论文

作者Zhang, Xiao-Lian4,5,6; Zhou, Xiang1; Long, Gang3; Li, Shu4,5,6; Fu, Bo-Shi1; Xu, Guo-Hua2; Wang, Xiao-Ning3; Xie, Yan4,5,6; Bian, Wen-Xiu4,5,6
刊名NUCLEIC ACIDS RESEARCH
出版日期2019-01-10
卷号47期号:1页码:56-68
ISSN号0305-1048
DOI10.1093/nar/gky1177
英文摘要Hepatitis C virus (HCV) infection is a major cause of human chronic liver disease and hepatocellular carcinoma. G-quadruplex (G4) is an important four-stranded secondary structure of nucleic acids. Recently, we discovered that the core gene of HCV contains a G4 RNA structure; however, the interaction between the HCV core RNA G4 and host cellular proteins, and the roles of the HCV core RNA G4 in HCV infection and pathogenesis remain elusive. Here, we identified a cellular protein, nucleolin (NCL), which bound and stabilized the HCV core RNA G4 structure. We demonstrated the direct interaction and colocalization between NCL and wild-type core RNA G4 at both in vitro and in cell physiological conditions of the alive virus; however no significant interaction was found between NCL and G4-modified core RNA. NCL is also associated with HCV particles. HCV infection induced NCL mRNA and protein expression, while NCL suppressed wild-type viral replication and expression, but not G4-modified virus. Silencing of NCL greatly enhanced viral RNA replication. Our findings provide new insights that NCL may act as a host factor for anti-viral innate immunity, and binding of cellular NCL with the viral core RNA G4 structure is involved in suppressing HCV replication.
WOS关键词HEPATITIS-C-VIRUS ; MYC G-QUADRUPLEX ; MESSENGER-RNA ; DNA ; PROMOTER ; PROTEIN ; IDENTIFICATION ; EXPRESSION ; INFECTION ; SELECTION
资助项目National Key R&D Program of China[2018YFA0507603] ; National Natural Science Foundation of China[21572173] ; National Natural Science Foundation of China[91740120] ; National Natural Science Foundation of China[31370197] ; National Natural Science Foundation of China[21432008] ; National Natural Science Foundation of China[21721005] ; National Natural Science Foundation of China[91753201] ; National Outstanding Youth Foundation of China[81025008] ; Major Projects of Technological Innovation of Hubei Province[2016ACA150] ; Natural Science Foundation Project of Hunan Province[2016CFA062]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000462586700012
出版者OXFORD UNIV PRESS
资助机构National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Outstanding Youth Foundation of China ; National Outstanding Youth Foundation of China ; Major Projects of Technological Innovation of Hubei Province ; Major Projects of Technological Innovation of Hubei Province ; Natural Science Foundation Project of Hunan Province ; Natural Science Foundation Project of Hunan Province ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Outstanding Youth Foundation of China ; National Outstanding Youth Foundation of China ; Major Projects of Technological Innovation of Hubei Province ; Major Projects of Technological Innovation of Hubei Province ; Natural Science Foundation Project of Hunan Province ; Natural Science Foundation Project of Hunan Province ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Outstanding Youth Foundation of China ; National Outstanding Youth Foundation of China ; Major Projects of Technological Innovation of Hubei Province ; Major Projects of Technological Innovation of Hubei Province ; Natural Science Foundation Project of Hunan Province ; Natural Science Foundation Project of Hunan Province ; National Key R&D Program of China ; National Key R&D Program of China ; National Natural Science Foundation of China ; National Natural Science Foundation of China ; National Outstanding Youth Foundation of China ; National Outstanding Youth Foundation of China ; Major Projects of Technological Innovation of Hubei Province ; Major Projects of Technological Innovation of Hubei Province ; Natural Science Foundation Project of Hunan Province ; Natural Science Foundation Project of Hunan Province
源URL[http://ir.wipm.ac.cn/handle/112942/13912]  
专题中国科学院武汉物理与数学研究所
通讯作者Zhang, Xiao-Lian; Zhou, Xiang
作者单位1.Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Wuhan Inst Phys & Math, Wuhan 430071, Hubei, Peoples R China
3.Chinese Acad Sci, Key Lab Mol Virol & Immunol, Inst Pasteur Shanghai, Shanghai Inst Biol Sci, Shanghai, Peoples R China
4.Wuhan Univ, Sch Basic Med Sci, Dept Immunol, Wuhan 430071, Peoples R China
5.Wuhan Univ, Sch Basic Med Sci, Hubei Prov Key Lab Allergy & Immunol, Med Res Inst, Wuhan 430071, Peoples R China
6.Wuhan Univ, Sch Basic Med Sci, State Key Lab Virol, Med Res Inst, Wuhan 430071, Peoples R China
推荐引用方式
GB/T 7714		 Zhang, Xiao-Lian,Zhou, Xiang,Long, Gang,et al. Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication[J]. NUCLEIC ACIDS RESEARCH,2019,47(1):56-68.
APA Zhang, Xiao-Lian.,Zhou, Xiang.,Long, Gang.,Li, Shu.,Fu, Bo-Shi.,...&Bian, Wen-Xiu.(2019).Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication.NUCLEIC ACIDS RESEARCH,47(1),56-68.
MLA Zhang, Xiao-Lian,et al."Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication".NUCLEIC ACIDS RESEARCH 47.1(2019):56-68.

入库方式： OAI收割

来源：武汉物理与数学研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。