Beyond the Roles in Biomimetic Chemistry: An Insight into the Intrinsic Catalytic Activity of an Enzyme for Tumor-Selective Phototheranostics
文献类型：期刊论文
| 作者 | Zhang, Bingbo3; Zhou, Xin1; Chen, Shizhen1; Yao, Defan3; Shi, Yuxin2; Shi, Xiudong2; Yang, Weitao3 |
| 刊名 | ACS NANO
 |
| 出版日期 | 2018-12-01 |
| 卷号 | 12期号:12页码:12169-12180 |
| 关键词 | biomimetic synthesis protein activity catalytic nanomedicine MR/PA imaging photothermal therapy |
| ISSN号 | 1936-0851 |
| DOI | 10.1021/acsnano.8b05797 |
| 英文摘要 | Protein-assisted biomimetic synthesis has been an emerging offshoot of nanofabrication in recent years owing to its features of green chemistry, facile process, and ease of multi-integration. As a result, many proteins have been used for biomimetic synthesis of varying kinds of nanostructures. Although the efforts on exploring new proteins and investigating their roles in biomimetic chemistry are increasing, the most essential intrinsic properties of proteins are largely neglected. Herein we report a frequently used enzyme (horseradish peroxidase, HRP) to demonstrate the possibility of enzymatic activity retaining after accomplishing the roles in biomimetic synthesis of ultrasmall gadolinium (Gd) nanodots and stowing its substrate 2,2'-Azinobis (3-ethylbenzothiazoline-6-sulfonic acid ammonium salt) (ABTS), denoted as Gd@HRPABTS. It was found that ca. 70% of the enzymatic activity of HRP was preserved. The associated changes of protein structure with chemical treatments were studied by spectroscopic analysis. Leveraging on the highly retained catalytic activity, Gd@HRPABTS exerts strong catalytic oxidation of peroxidase substrate ABTS into photoactive counterparts in the presence of intrinsic H2O2 inside the tumor, therefore enabling tumor-selective catalytic photoacoustic (PA) imaging and photothermal therapy (PTT). In addition, the MR moiety of Gd@HRPABTS provides guidance for PTT and further diagrams that Gd@HRPABTS is clearable from the body via kidneys. Preliminary toxicity studies show no observed adverse effects by administration of them. This study demonstrates beyond the well-known roles in biomimetic chemistry that HRP can also preserve its enzymatic activity for tumor catalytic theranostics. |
| WOS关键词 | MRI CONTRAST AGENTS ; NANOPARTICLES ; NANOCRYSTALS ; NANODOTS |
| 资助项目 | National Natural Science Foundation of China - China Postdoctoral Science Foundation[81801823] ; National Natural Science Foundation of China - China Postdoctoral Science Foundation[81571742] ; National Natural Science Foundation of China - China Postdoctoral Science Foundation[81871399] ; National Natural Science Foundation of China - China Postdoctoral Science Foundation[1500229020] ; Shanghai Skin Disease Hospital ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, CAS[T151701] ; Fundamental Research Funds for the Central Universities |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000454567500042 |
| 出版者 | AMER CHEMICAL SOC |
| 资助机构 | National Natural Science Foundation of China - China Postdoctoral Science Foundation ; National Natural Science Foundation of China - China Postdoctoral Science Foundation ; Shanghai Skin Disease Hospital ; Shanghai Skin Disease Hospital ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, CAS ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, CAS ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China - China Postdoctoral Science Foundation ; National Natural Science Foundation of China - China Postdoctoral Science Foundation ; Shanghai Skin Disease Hospital ; Shanghai Skin Disease Hospital ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, CAS ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, CAS ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China - China Postdoctoral Science Foundation ; National Natural Science Foundation of China - China Postdoctoral Science Foundation ; Shanghai Skin Disease Hospital ; Shanghai Skin Disease Hospital ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, CAS ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, CAS ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities ; National Natural Science Foundation of China - China Postdoctoral Science Foundation ; National Natural Science Foundation of China - China Postdoctoral Science Foundation ; Shanghai Skin Disease Hospital ; Shanghai Skin Disease Hospital ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, CAS ; State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Institute of Physics and Mathematics, CAS ; Fundamental Research Funds for the Central Universities ; Fundamental Research Funds for the Central Universities |
| 源URL | [http://ir.wipm.ac.cn/handle/112942/13976]  |
| 专题 | 中国科学院武汉物理与数学研究所 |
| 通讯作者 | Zhang, Bingbo |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Magnet Resonance Biol Syst, State Key Lab Magnet Resonance & Atom & Mol Phys, Natl Ctr Magnet Resonance Wuhan,Wuhan Inst Phys &, Wuhan 430071, Hubei, Peoples R China 2.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Dept Radiol, Shanghai 201508, Peoples R China 3.Tongji Univ, Sch Med, Inst Biomed Engn & Nano Sci, Inst Photomed,Shanghai Skin Dis Hosp, Shanghai 200443, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Bingbo,Zhou, Xin,Chen, Shizhen,et al. Beyond the Roles in Biomimetic Chemistry: An Insight into the Intrinsic Catalytic Activity of an Enzyme for Tumor-Selective Phototheranostics[J]. ACS NANO,2018,12(12):12169-12180. |
| APA | Zhang, Bingbo.,Zhou, Xin.,Chen, Shizhen.,Yao, Defan.,Shi, Yuxin.,...&Yang, Weitao.(2018).Beyond the Roles in Biomimetic Chemistry: An Insight into the Intrinsic Catalytic Activity of an Enzyme for Tumor-Selective Phototheranostics.ACS NANO,12(12),12169-12180. |
| MLA | Zhang, Bingbo,et al."Beyond the Roles in Biomimetic Chemistry: An Insight into the Intrinsic Catalytic Activity of an Enzyme for Tumor-Selective Phototheranostics".ACS NANO 12.12(2018):12169-12180. |
入库方式： OAI收割
来源：武汉物理与数学研究所
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