Structural analysis of Wss1 protein from saccharomyces cerevisiae
文献类型：期刊论文
| 作者 | Wang, WJ ; Dong, YH; Xu, JH; Li, ZQ; Gao, ZQ; Li, YH; Dong YH(董宇辉); Xu JH(徐建华); Gao ZQ(高增强); Li YH(李艳华)
|
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2017 |
| 卷号 | 7页码:8270 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-017-08834-w |
| 文献子类 | Article |
| 英文摘要 | Wss1 is a DNA-protein crosslinks (DPCs) repair protein, which is responsible for degradation of the protein components in DPCs. In this investigation, crystal structure of the protease domain from saccharomyces cerevisiae Wss1 (ScWss1) was solved and was compared with the known crystal structure of Schizosaccharomyces prombe Wss1 (SpWss1). It is found that the cleft near zinc ion to be the most conserved core region of Wss1 and that the electronic surface distributions vary greatly between the two homologs. Solution architecture of the full-length ScWss1 was further investigated by small-angle X-ray scattering (SAXS), which indicated the protein contains a flexible region inside. Finally, based on the structural information, a mechanism was proposed about how the enzyme is activated by DNA substrates. |
| WOS关键词 | CROSS-LINK REPAIR ; DNA-REPAIR ; SCATTERING ; SYSTEM |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000407570000147 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/284863]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Wang, WJ,Dong, YH,Xu, JH,et al. Structural analysis of Wss1 protein from saccharomyces cerevisiae[J]. SCIENTIFIC REPORTS,2017,7:8270. |
| APA | Wang, WJ.,Dong, YH.,Xu, JH.,Li, ZQ.,Gao, ZQ.,...&Yang, XY.(2017).Structural analysis of Wss1 protein from saccharomyces cerevisiae.SCIENTIFIC REPORTS,7,8270. |
| MLA | Wang, WJ,et al."Structural analysis of Wss1 protein from saccharomyces cerevisiae".SCIENTIFIC REPORTS 7(2017):8270. |
入库方式： OAI收割
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。