Interrogating the variation of element masses and distribution patterns in single cells using ICP-MS with a high efficiency cell introduction system
文献类型：期刊论文
| 作者 | Wang, M ; Wang HL(王海龙); Wang M(王萌); Wang B(汪冰); Zheng LN(郑令娜); Chen HQ(陈汉清); Chai ZF(柴之芳); Feng WY(丰伟悦); Wang, HL; Wang, B
|
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2017 |
| 卷号 | 409期号:5页码:1415-1423 |
| 关键词 | Single-cell analysis Single-cell ICP-MS Human cell lines Endogenous elements Variation Subpopulation |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-016-0075-y |
| 文献子类 | Article |
| 英文摘要 | Cellular heterogeneity is an inherent condition of cell populations, which results from stochastic expression of genes, proteins, and metabolites. The heterogeneity of individual cells can dramatically influence cellular decision-making and cell fate. So far, our knowledge about how the variation of endogenous metals and non-metals in individual eukaryotic cells is limited. In this study, ICP-MS equipped with a high efficiency cell introduction system (HECIS) was developed as a method of single-cell ICP-MS (SC-ICP-MS). The method was applied to the single-cell analysis of Mn, Fe, Co, Cu, Zn, P, and S in human cancer cell lines (HeLa and A549) and normal human bronchial epithelial cell line (16HBE). The analysis showed obvious variation of the masses of Cu, Fe, Zn, and P in individual HeLa cells, and variation of Fe, Zn, and P in individual A549 cells. On the basis of the single-cell data, a multimodal distribution of the elements in the cell population was fitted, which showed marked differences among the various cell lines. Importantly, subpopulations of the elements were found in the cell populations, especially in the HeLa cancer cells. This study demonstrates that SC-ICP-MS is able to unravel the extent of variation of endogenous elements in individual cells, which will help to improve our fundamental understanding of cellular biology and reveal novel insights into human biology and medicine. |
| 电子版国际标准刊号 | 1618-2650 |
| WOS关键词 | ATOMIC EMISSION-SPECTROMETRY ; SACCHAROMYCES-CEREVISIAE ; TRACE-ELEMENTS ; CYTOMETRY ; CANCER ; NANOPARTICLES ; PROGRESSION ; EXPRESSION ; NEBULIZER ; CYCLE |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000392622800023 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/284946]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Wang, M,Wang HL,Wang M,et al. Interrogating the variation of element masses and distribution patterns in single cells using ICP-MS with a high efficiency cell introduction system[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2017,409(5):1415-1423. |
| APA | Wang, M.,王海龙.,王萌.,汪冰.,郑令娜.,...&Feng, WY.(2017).Interrogating the variation of element masses and distribution patterns in single cells using ICP-MS with a high efficiency cell introduction system.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,409(5),1415-1423. |
| MLA | Wang, M,et al."Interrogating the variation of element masses and distribution patterns in single cells using ICP-MS with a high efficiency cell introduction system".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 409.5(2017):1415-1423. |
入库方式： OAI收割
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。