中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Reaction-Based Off On Near-infrared Fluorescent Probe for Imaging Alkaline Phosphatase Activity in Living Cells and Mice

文献类型:期刊论文

作者Ho, D; Yao, SQ; Hu, Y; Hu Y(胡毅); Sun, HY; Tan, Y; Zhang, L; Man, KH; Peltier, R; Chen, GC
刊名ACS APPLIED MATERIALS & INTERFACES
出版日期2017
卷号9期号:8页码:6796-6803
关键词alkaline phosphatase near-infrared fluorescent probe bioimaging mice
ISSN号1944-8244
DOI10.1021/acsami.6b14176
文献子类Article
英文摘要Alkaline phosphatases are a group of enzymes that play important roles in regulating diverse cellular functions and disease pathogenesis. Hence, developing fluorescent probes for in vivo detection of alkaline phosphatase activity is highly desirable for studying the dynamic phosphorylation in living organisms. Here, we developed the very first reaction based near-infrared (NIR) probe (DHXP) for sensitive detection of alkaline phosphatase activity both in vitro and in vivo. Our studies demonstrated that the probe displayed an up to 66-fold fluorescence increment upon incubation with alkaline phosphatases, and the detection limit of our probe was determined to be 0.07 U/L, which is lower than that of most of alkaline phosphatase probes reported in literature. Furthermore, we demonstrated that the probe can be applied to detecting alkaline phosphatase activity in cells and mice. In addition, our probe possesses excellent biocompatibility and rapid cell-internalization ability. In light of these prominent properties, we envision that DHXP will add useful tools for investigating alkaline phosphatase activity in biomedical research.
WOS关键词PROTEIN-TYROSINE PHOSPHATASES ; AGGREGATION-INDUCED EMISSION ; IN-VIVO ; SELECTIVE DETECTION ; ON PROBE ; TRACKING ; ASSAY ; NANOPARTICLES ; DYE ; TETRAPHENYLETHYLENE
WOS研究方向Science & Technology - Other Topics ; Materials Science
语种英语
WOS记录号WOS:000395494200014
源URL[http://ir.ihep.ac.cn/handle/311005/285130]  
专题高能物理研究所_粒子天体物理中心
高能物理研究所_实验物理中心
高能物理研究所_加速器中心
高能物理研究所_多学科研究中心
作者单位中国科学院高能物理研究所
推荐引用方式
GB/T 7714
Ho, D,Yao, SQ,Hu, Y,et al. Reaction-Based Off On Near-infrared Fluorescent Probe for Imaging Alkaline Phosphatase Activity in Living Cells and Mice[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(8):6796-6803.
APA Ho, D.,Yao, SQ.,Hu, Y.,胡毅.,Sun, HY.,...&Wang, F.(2017).Reaction-Based Off On Near-infrared Fluorescent Probe for Imaging Alkaline Phosphatase Activity in Living Cells and Mice.ACS APPLIED MATERIALS & INTERFACES,9(8),6796-6803.
MLA Ho, D,et al."Reaction-Based Off On Near-infrared Fluorescent Probe for Imaging Alkaline Phosphatase Activity in Living Cells and Mice".ACS APPLIED MATERIALS & INTERFACES 9.8(2017):6796-6803.

入库方式: OAI收割

来源:高能物理研究所

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