Reaction-Based Off On Near-infrared Fluorescent Probe for Imaging Alkaline Phosphatase Activity in Living Cells and Mice
文献类型：期刊论文
| 作者 | Ho, D; Yao, SQ; Hu, Y ; Hu Y(胡毅); Sun, HY; Tan, Y; Zhang, L; Man, KH; Peltier, R; Chen, GC
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| 刊名 | ACS APPLIED MATERIALS & INTERFACES
 |
| 出版日期 | 2017 |
| 卷号 | 9期号:8页码:6796-6803 |
| 关键词 | alkaline phosphatase near-infrared fluorescent probe bioimaging mice |
| ISSN号 | 1944-8244 |
| DOI | 10.1021/acsami.6b14176 |
| 文献子类 | Article |
| 英文摘要 | Alkaline phosphatases are a group of enzymes that play important roles in regulating diverse cellular functions and disease pathogenesis. Hence, developing fluorescent probes for in vivo detection of alkaline phosphatase activity is highly desirable for studying the dynamic phosphorylation in living organisms. Here, we developed the very first reaction based near-infrared (NIR) probe (DHXP) for sensitive detection of alkaline phosphatase activity both in vitro and in vivo. Our studies demonstrated that the probe displayed an up to 66-fold fluorescence increment upon incubation with alkaline phosphatases, and the detection limit of our probe was determined to be 0.07 U/L, which is lower than that of most of alkaline phosphatase probes reported in literature. Furthermore, we demonstrated that the probe can be applied to detecting alkaline phosphatase activity in cells and mice. In addition, our probe possesses excellent biocompatibility and rapid cell-internalization ability. In light of these prominent properties, we envision that DHXP will add useful tools for investigating alkaline phosphatase activity in biomedical research. |
| WOS关键词 | PROTEIN-TYROSINE PHOSPHATASES ; AGGREGATION-INDUCED EMISSION ; IN-VIVO ; SELECTIVE DETECTION ; ON PROBE ; TRACKING ; ASSAY ; NANOPARTICLES ; DYE ; TETRAPHENYLETHYLENE |
| WOS研究方向 | Science & Technology - Other Topics ; Materials Science |
| 语种 | 英语 |
| WOS记录号 | WOS:000395494200014 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/285130]  |
| 专题 | 高能物理研究所_粒子天体物理中心 高能物理研究所_实验物理中心 高能物理研究所_加速器中心 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Ho, D,Yao, SQ,Hu, Y,et al. Reaction-Based Off On Near-infrared Fluorescent Probe for Imaging Alkaline Phosphatase Activity in Living Cells and Mice[J]. ACS APPLIED MATERIALS & INTERFACES,2017,9(8):6796-6803. |
| APA | Ho, D.,Yao, SQ.,Hu, Y.,胡毅.,Sun, HY.,...&Wang, F.(2017).Reaction-Based Off On Near-infrared Fluorescent Probe for Imaging Alkaline Phosphatase Activity in Living Cells and Mice.ACS APPLIED MATERIALS & INTERFACES,9(8),6796-6803. |
| MLA | Ho, D,et al."Reaction-Based Off On Near-infrared Fluorescent Probe for Imaging Alkaline Phosphatase Activity in Living Cells and Mice".ACS APPLIED MATERIALS & INTERFACES 9.8(2017):6796-6803. |
入库方式： OAI收割
来源：高能物理研究所
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