Phase behavior of a binary lipid system containing long- and short-chain phosphatidylcholines
文献类型：期刊论文
| 作者 | Yu, ZW; Li ZH(李志宏) ; Li, ZH; Wu, FG; Sun, HY; Deng, G; Zhou, Y
|
| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2017 |
| 卷号 | 7期号:10页码:5715-5724 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c6ra24961b |
| 文献子类 | Article |
| 英文摘要 | Bilayered micelles, or so-called bicelles, are generally made of long-and short-chain lipids. They are extensively used as model membranes to study the structure of membrane-associated peptides or proteins and their interactions with membranes. However, the phase behavior of lipid mixtures composed of longand short-chain lipids, especially at low temperatures, is still not very clear. In this work, the most commonly used long-chain lipid, 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), and a short-chain lipid, 1,2-dioctanoyl-sn-glycero-3-phosphocholine (diC8PC), were selected as a bicellar model to study their phase behavior. Over the whole range of DPPC/diC8PC molar ratios (q) studied in this work, a lamellar crystalline phase (L-c') enriched in DPPC was found to be the most stable phase at 5 degrees C, together with a diC8PC-enriched micelle phase. Interestingly, a metastable phase, named the U phase in this work, was observed in the mixtures with a DPPC/diC8PC molar ratio between 1 and 4. The metastable U phase was found to be lacking in long-range order in the direction of the bilayer surface normal, but bearing a different "crystalline phase-like" hydrocarbon chain packing mode, in comparison with the lamellar crystalline phase. The kinetic properties of the U phase were also studied in detail, and it was found that the phase acts as a precursor phase in the process of forming themost stable crystalline phase. This work deepens our understanding of lipid crystallization behavior, and is also a step forward towards a more detailed picture of the phase behavior of lipid mixtures composed of long-and short-chain lipids. |
| WOS关键词 | X-RAY-DIFFRACTION ; SOLID-STATE NMR ; HYDRATED DIPALMITOYLPHOSPHATIDYLCHOLINE BILAYERS ; NUCLEAR-MAGNETIC-RESONANCE ; METASTABLE SUBGEL PHASE ; MEMBRANE-PROTEINS ; SATURATED DIACYLPHOSPHATIDYLCHOLINES ; MODEL MEMBRANES ; BICELLES ; SPECTROSCOPY |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000393754100021 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/285169]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Yu, ZW,Li ZH,Li, ZH,et al. Phase behavior of a binary lipid system containing long- and short-chain phosphatidylcholines[J]. RSC ADVANCES,2017,7(10):5715-5724. |
| APA | Yu, ZW.,李志宏.,Li, ZH.,Wu, FG.,Sun, HY.,...&Zhou, Y.(2017).Phase behavior of a binary lipid system containing long- and short-chain phosphatidylcholines.RSC ADVANCES,7(10),5715-5724. |
| MLA | Yu, ZW,et al."Phase behavior of a binary lipid system containing long- and short-chain phosphatidylcholines".RSC ADVANCES 7.10(2017):5715-5724. |
入库方式： OAI收割
来源：高能物理研究所
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