Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination
文献类型：期刊论文
| 作者 | Shu, D; Montemagno, C; Ding, BQ; Li, JG ; Guo, PX; Li JY(李敬源); Zhang, H; Zhao, Z
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| 刊名 | SMALL
 |
| 出版日期 | 2017 |
| 卷号 | 13期号:1页码:1601600 |
| ISSN号 | 1613-6810 |
| DOI | 10.1002/smll.201601600 |
| 文献子类 | Article |
| 英文摘要 | The significance of bionanomotors in nanotechnology is analogous to mechanical motors in daily life. Here the principle and approach for designing and constructing biomimetic nanomotors with continuous single-directional motion are reported. This bionanomotor is composed of a dodecameric protein channel, a six-pRNA ring, and an ATPase hexamer. Based on recent elucidations of the one-way revolving mechanisms of the phi29 double-stranded DNA (dsDNA) motor, various RNA and protein elements are designed and tested by single-molecule imaging and biochemical assays, with which the motor with active components has been constructed. The motor motion direction is controlled by three operation elements: (1) Asymmetrical ATPase with ATP-interacting domains for alternative DNA binding/pushing regulated by an arginine finger in a sequential action manner. The arginine finger bridges two adjacent ATPase subunits into a non-covalent dimer, resulting in an asymmetrical hexameric complex containing one dimer and four monomers. (2) The dsDNA translocation channel as a one-way valve. (3) The hexameric pRNA ring geared with left-/right-handed loops. Assessments of these constructs reveal that one inactive subunit of pRNA/ATPase is sufficient to completely block motor function (defined as K = 1), implying that these components work sequentially based on the principle of binomial distribution and Yang Hui's triangle. |
| 电子版国际标准刊号 | 1613-6829 |
| WOS关键词 | DNA-PACKAGING MOTOR ; WAY VALVE MECHANISM ; ARGININE FINGER ; BACTERIOPHAGE-PHI-29 DNA ; BACILLUS-SUBTILIS ; ATPASE ACTIVITY ; NUCLEIC-ACIDS ; AAA+ PROTEINS ; PHAGE PHI-29 ; GENOMIC DNA |
| WOS研究方向 | Chemistry ; Science & Technology - Other Topics ; Materials Science ; Physics |
| 语种 | 英语 |
| WOS记录号 | WOS:000396562400004 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/285183]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Shu, D,Montemagno, C,Ding, BQ,et al. Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination[J]. SMALL,2017,13(1):1601600. |
| APA | Shu, D.,Montemagno, C.,Ding, BQ.,Li, JG.,Guo, PX.,...&Zhao, Z.(2017).Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination.SMALL,13(1),1601600. |
| MLA | Shu, D,et al."Construction of Asymmetrical Hexameric Biomimetic Motors with Continuous Single-Directional Motion by Sequential Coordination".SMALL 13.1(2017):1601600. |
入库方式： OAI收割
来源：高能物理研究所
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