Integrative approach for the analysis of the proteome-wide response to bismuth drugs in Helicobacter pylori
文献类型：期刊论文
| 作者 | Wang, YC; Hu, LG; Xu, F; Quan, Q; Lai, YT; Xia, W; Yang, Y; Chang, YY; Yang, XM; Chai, ZF
|
| 刊名 | CHEMICAL SCIENCE
 |
| 出版日期 | 2017 |
| 卷号 | 8期号:6页码:4626-4633 |
| ISSN号 | 2041-6520 |
| DOI | 10.1039/c7sc00766c |
| 文献子类 | Article |
| 英文摘要 | Bismuth drugs, despite being clinically used for decades, surprisingly remain in use and effective for the treatment of Helicobacter pylori infection, even for resistant strains when co-administrated with antibiotics. However, the molecular mechanisms underlying the clinically sustained susceptibility of H. pylori to bismuth drugs remain elusive. Herein, we report that integration of in-house metalloproteomics and quantitative proteomics allows comprehensive uncovering of the bismuth-associated proteomes, including 63 bismuth-binding and 119 bismuth-regulated proteins from Helicobacter pylori, with over 60% being annotated with catalytic functions. Through bioinformatics analysis in combination with bioassays, we demonstrated that bismuth drugs disrupted multiple essential pathways in the pathogen, including ROS defence and pH buffering, by binding and functional perturbation of a number of key enzymes. Moreover, we discovered that HpDnaK may serve as a new target of bismuth drugs to inhibit bacterium-host cell adhesion. The integrative approach we report, herein, provides a novel strategy to unveil the molecular mechanisms of antimicrobial metals against pathogens in general. This study sheds light on the design of new types of antimicrobial agents with multiple targets to tackle the current crisis of antimicrobial resistance. |
| 电子版国际标准刊号 | 2041-6539 |
| WOS关键词 | METAL AFFINITY-CHROMATOGRAPHY ; BINDING PROTEINS ; MASS-SPECTROMETRY ; CELLS ; IDENTIFICATION ; RESISTANCE ; NETWORKS ; METALLOPROTEOMICS ; SUSCEPTIBILITY ; ERADICATION |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000402384900056 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/285210]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_加速器中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Wang, YC,Hu, LG,Xu, F,et al. Integrative approach for the analysis of the proteome-wide response to bismuth drugs in Helicobacter pylori[J]. CHEMICAL SCIENCE,2017,8(6):4626-4633. |
| APA | Wang, YC.,Hu, LG.,Xu, F.,Quan, Q.,Lai, YT.,...&柴之芳.(2017).Integrative approach for the analysis of the proteome-wide response to bismuth drugs in Helicobacter pylori.CHEMICAL SCIENCE,8(6),4626-4633. |
| MLA | Wang, YC,et al."Integrative approach for the analysis of the proteome-wide response to bismuth drugs in Helicobacter pylori".CHEMICAL SCIENCE 8.6(2017):4626-4633. |
入库方式： OAI收割
来源：高能物理研究所
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