热门
Au nanoclusters suppress chronic lymphocytic leukaemia cells by inhibiting thioredoxin reductase 1 to induce intracellular oxidative stress and apoptosis
文献类型：期刊论文
| 作者 | Zhao, M ; Gao, L; Liu, R; Wang, YL; Gong, Y; Gao, FP; Gao, XY; Gong Y(龚勇); Li, Q; Yuan, Q
|
| 刊名 | SCIENCE BULLETIN科学通报
 |
| 出版日期 | 2017 |
| 卷号 | 62期号:8页码:537-545 |
| 关键词 | Chronic lymphocytic leukaemia (CLL) Gold nanoclusters Thioredoxin reductase (TrxR) Mitochondrial membrane potential (MMP) Apoptosis |
| ISSN号 | 2095-9273 |
| DOI | 10.1016/j.scib.2017.03.024 |
| 文献子类 | Article |
| 英文摘要 | Chronic lymphocytic leukaemia (CLL) is a rare blood cancer that always relapses as refractory disease and eventually leads to death. To date, therapeutic options for CLL patients are scarce and there is an urgent need to develop novel chemotherapeutics that are both effective and safe. Gold-containing compounds induce a lethal oxidative and endoplasmic reticulum stress response in cultured and primary CLL cells via inhibition of thioredoxin reductase (TrxR). However, traditional gold-containing medicines have revealed side effects during clinical applications. Therefore, safer gold-containing drugs are needed to overcome this challenge. In this study, a novel peptide templated gold cluster Au25Sv9 was synthesized and its therapeutic effect on CLL cells was evaluated. This nanocluster could induce cell apoptosis in MEC-1 cells in a dose-dependent manner which correlated with the uptake amount of clusters in cells. As expected, increasing intracellular reactive oxidative species (ROS) in MEC-1 cells was exhibited with the increase of cluster dosage. Further analyses demonstrated the underlying mechanism that the nanoclusters suppress the activity of TrxR1, increase the level of intracellular ROS, destroy the mitochondrial membrane potential and finally trigger the mitochondrial apoptotic pathway in MEC-1 cells. Furthermore, the direct interaction between Au25Sv9 clusters and TrxR1 was confirmed for the first time by isothermal titration calorimetry. These findings explored the preclinical efficacy and potential mechanism of gold clusters in CLL therapy and provided a fundamental reference for the development of other novel gold-containing chemotherapeutics to treat CLL. (C) 2017 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved. |
| 电子版国际标准刊号 | 2095-9281 |
| WOS关键词 | BIOMEDICAL APPLICATIONS ; RHEUMATOID-ARTHRITIS ; CYTOTOXIC SECRETIONS ; AURANOFIN ; GOLD ; DRUG ; MECHANISM ; CANCER ; TUMOR ; TRXR1 |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| CSCD记录号 | CSCD:6006884 |
| WOS记录号 | WOS:000403561400003 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/285220]  |
| 专题 | 高能物理研究所_多学科研究中心 高能物理研究所_实验物理中心 高能物理研究所_粒子天体物理中心 高能物理研究所_管理与技术支持 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Zhao, M,Gao, L,Liu, R,et al. Au nanoclusters suppress chronic lymphocytic leukaemia cells by inhibiting thioredoxin reductase 1 to induce intracellular oxidative stress and apoptosis[J]. SCIENCE BULLETIN科学通报,2017,62(8):537-545. |
| APA | Zhao, M.,Gao, L.,Liu, R.,Wang, YL.,Gong, Y.,...&Yao, YW.(2017).Au nanoclusters suppress chronic lymphocytic leukaemia cells by inhibiting thioredoxin reductase 1 to induce intracellular oxidative stress and apoptosis.SCIENCE BULLETIN科学通报,62(8),537-545. |
| MLA | Zhao, M,et al."Au nanoclusters suppress chronic lymphocytic leukaemia cells by inhibiting thioredoxin reductase 1 to induce intracellular oxidative stress and apoptosis".SCIENCE BULLETIN科学通报 62.8(2017):537-545. |
入库方式： OAI收割
来源：高能物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。