Evaluating the toxicity of silicon dioxide nanoparticles on neural stem cells using RNA-Seq
文献类型：期刊论文
| 作者 | Gu, ZJ ; Sun, DY; Gong LJ(龚林吉); Gong, LJ; Xie, J; He, X; Chen, SY; Gu ZJ(谷战军); Luodan, A; Li, QY
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| 刊名 | RSC ADVANCES
 |
| 出版日期 | 2017 |
| 卷号 | 7期号:75页码:47552-47564 |
| ISSN号 | 2046-2069 |
| DOI | 10.1039/c7ra09512k |
| 文献子类 | Article |
| 英文摘要 | Neural stem cells are characterized by self-renewal and multipotency, and a capacity to regenerate in response to brain injury or neurodegenerative disease. Silicon dioxide nanoparticles (SiO2 NPs) are novel materials, which enable the delivery of specific payloads to stem cells; for example, genes or proteins, to enable cell-fate manipulation, or tracer materials, to enable in vivo tracing. However, little is known about the dose-dependent cytotoxicity of SiO2 NPs, and how exposure to SiO2 NPs changes mRNA expression profiles in neural stem cells. In this study, a mouse C17.2 neural stem cell line was treated with 90 nm monodisperse fluorescein isothiocyanate-SiO2 NPs at 0, 100, 200 and 400 mg mL(-1) for 48 hours. Internalization of SiO2 NPs was observed in C17.2 cells in a dose-dependent manner. SiO2 NP exposure induced apoptosis and inhibited cell proliferation in the C17.2 cell line at dosage levels of 200 mg mL(-1) and above. Microscopically, mitochondrial swelling and cristae fracture were observed. Furthermore, next generation RNA sequencing (RNA-Seq) indicated that high-dose SiO2 NP exposure specifically inhibited transcription of glutathione-S-transferase (GST) genes, including GSTM1, GSTM7 and GSTT1. These results suggest that application of high-dose SiO2 NPs to the nervous system may cause neurotoxicity, induce apoptosis and reduce neural stem cell proliferation by inhibiting GST gene expression. |
| WOS关键词 | BLOOD-BRAIN-BARRIER ; DRUG-DELIVERY ; IN-VIVO ; NEURODEGENERATIVE DISEASES ; OXIDE NANOPARTICLES ; EPITHELIAL-CELLS ; GENE DELIVERY ; PROLIFERATION ; EXPRESSION ; REVEALS |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000412977000039 |
| 源URL | [http://ir.ihep.ac.cn/handle/311005/285297]  |
| 专题 | 高能物理研究所_多学科研究中心 |
| 作者单位 | 中国科学院高能物理研究所 |
| 推荐引用方式 GB/T 7714 | Gu, ZJ,Sun, DY,Gong LJ,et al. Evaluating the toxicity of silicon dioxide nanoparticles on neural stem cells using RNA-Seq[J]. RSC ADVANCES,2017,7(75):47552-47564. |
| APA | Gu, ZJ.,Sun, DY.,龚林吉.,Gong, LJ.,Xie, J.,...&Xu, HW.(2017).Evaluating the toxicity of silicon dioxide nanoparticles on neural stem cells using RNA-Seq.RSC ADVANCES,7(75),47552-47564. |
| MLA | Gu, ZJ,et al."Evaluating the toxicity of silicon dioxide nanoparticles on neural stem cells using RNA-Seq".RSC ADVANCES 7.75(2017):47552-47564. |
入库方式： OAI收割
来源：高能物理研究所
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