Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli
文献类型：期刊论文
| 作者 | Liu, Min1,2; Ding, Yamei3; Xian, Mo1; Zhao, Guang1,2 |
| 刊名 | MICROBIAL CELL FACTORIES
 |
| 出版日期 | 2018-03-28 |
| 卷号 | 17页码:11 |
| 关键词 | Xylose Glycolate Glycolaldehyde Glyoxylate Escherichia coli |
| ISSN号 | 1475-2859 |
| DOI | 10.1186/s12934-018-0900-4 |
| 通讯作者 | Xian, Mo(xianmo1@qibebt.ac.cn) ; Zhao, Guang(zhaoguang@qibebt.ac.cn) |
| 英文摘要 | Background: Glycolate is a valuable chemical with extensive applications in many different fields. The traditional methods to synthesize glycolate are quite expensive and toxic. So, the biotechnological production of glycolate from sustainable feedstocks is of interest for its potential economic and environmental advantages. D-Xylose is the second most abundant sugar in nature and accounts for 18-30% of sugar in lignocellulose. New routes for the conversion of xylose to glycolate were explored. Results: Overexpression of aceA and ghrA and deletion of aceB in Escherichia coli were examined for glycolate production from xylose, but the conversion was initially ineffective. Then, a new route for glycolate production was established in E. coli by introducing-NAD(+)-dependent xylose dehydrogenase (xdh) and xylonolactonase (xylC) from Caulobacter crescentus. The constructed engineered strain Q2562 produced 28.82 +/- 0.56 g/L glycolate from xylose with 0.60 +/- 0.01 g/L/h productivity and 0.38 +/- 0.07 g/g xylose yield. However, 27.18 +/- 2.13 g/L acetate was accumulated after fermentation. Deletions of iclR and ackA were used to overcome the acetate excretion. An ackA knockout resulted in about 66% decrease in acetate formation. The final engineered strain Q2742 produced 43.60 +/- 1.22 g/L glycolate, with 0.91 +/- 0.02 g/L/h productivity and 0.46 +/- 0.03 g/g xylose yield. Conclusions: A new route for glycolate production from xylose was established, and an engineered strain Q2742 was constructed from this new explored pathway. The engineering strain showed the highest reported productivity of glycolate to date. This research opened up a new prospect for bio-refinery of xylose and an alternative choice for industrial production of glycolate. |
| 资助项目 | National Natural Science Foundation of China[31670089] ; National Natural Science Foundation of China[31722001] ; China Postdoctoral Science Foundation[2016M600564] ; Postdoctoral Applied Research Project of Qingdao |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000428910100002 |
| 出版者 | BIOMED CENTRAL LTD |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/158450]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Xian, Mo; Zhao, Guang |
| 作者单位 | 1.Chinese Acad Siences, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, Qingdao 266101, Peoples R China 2.Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Min,Ding, Yamei,Xian, Mo,et al. Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli[J]. MICROBIAL CELL FACTORIES,2018,17:11. |
| APA | Liu, Min,Ding, Yamei,Xian, Mo,&Zhao, Guang.(2018).Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli.MICROBIAL CELL FACTORIES,17,11. |
| MLA | Liu, Min,et al."Metabolic engineering of a xylose pathway for biotechnological production of glycolate in Escherichia coli".MICROBIAL CELL FACTORIES 17(2018):11. |
入库方式： OAI收割
来源：海洋研究所
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