中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 海洋研究所
  3. 中国科学院海洋研究所
Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas

文献类型：期刊论文

作者Li, Chunyan1,2,3,5,6; Wang, Jinpeng1,2,3,5,6; Song, Kai1,3,5,6; Meng, Jie1,3,5,6; Xu, Fei1,3,5,6; Li, Li1,4,5,6; Zhang, Guofan1,3,5,6
刊名BMC GENOMICS
出版日期2018-08-22
卷号19页码:15
关键词Crassostrea gigas Genetic map Collinearity Quantitative trait loci (QTL) mapping Growth Nutrition
ISSN号1471-2164
DOI10.1186/s12864-018-4996-z
通讯作者Li, Li(lili@qdio.ac.cn) ; Zhang, Guofan(gfzhang@qdio.ac.cn)
英文摘要Background: Both growth and nutritional traits are important economic traits of Crassostrea gigas (C. gigas) in industry. But few work has been done to study the genetic architecture of nutritional traits of the oyster. In this study, we constructed a high-density genetic map of C. gigas to help assemble the genome sequence onto chromosomes, meanwhile explore the genetic basis for nutritional traits via quantitative trait loci (QTL) mapping. Results: The constructed genetic map contained 5024 evenly distributed markers, with an average marker interval of 0.68 cM, thus representing the densest genetic map produced for the oyster. According to the high collinearity between the consensus map and the oyster genome, 1574 scaffold (about 70%) of the genome sequence of C. gigas were successfully anchored to 10 linkage groups (LGs) of the consensus map. Using this high-qualified genetic map, we then conducted QTL analysis for growth and nutritional traits, the latter of which includes glycogen, amino acid (AA), and fatty acid (FA). Overall, 41 QTLs were detected for 17 traits. In addition, six candidate genes identified in the QTL interval showed significant correlation with the traits on transcriptional levels. These genes include growth-related genes AMY and BMP1, AA metabolism related genes PLSCR and GR, and FA metabolism regulation genes DYRK and ADAMTS. Conclusion: Using the constructed high-qualified linkage map, this study not only assembled nearly 70% of the oyster genome sequence onto chromosomes, but also identified valuable markers and candidate genes for growth and nutritional traits, especially for AA and FA that undergone few studies before. These findings will facilitate genome assembly and molecular breeding of important economic traits in C. gigas.
资助项目National Natural Science Foundation of China[31530079] ; National Natural Science Foundation of China[31572620] ; Strategic Priority Research Program of Western Pacific Ocean System: Structure, Dynamics and Consequences[XDA586 11020305] ; Earmarked Fund for Modern Agro-industry Technology Research System[CARS-49]
WOS研究方向Biotechnology & Applied Microbiology ; Genetics & Heredity
语种英语
WOS记录号WOS:000442532000005
出版者BMC
源URL[http://ir.qdio.ac.cn/handle/337002/159886]  
专题中国科学院海洋研究所
通讯作者Li, Li; Zhang, Guofan
作者单位1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries & Aquaculture, Qingdao, Peoples R China
5.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
6.Chinese Acad Sci, Inst Oceanol, Natl & Local Joint Engn Lab Ecol Mariculture, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Li, Chunyan,Wang, Jinpeng,Song, Kai,et al. Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas[J]. BMC GENOMICS,2018,19:15.
APA Li, Chunyan.,Wang, Jinpeng.,Song, Kai.,Meng, Jie.,Xu, Fei.,...&Zhang, Guofan.(2018).Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas.BMC GENOMICS,19,15.
MLA Li, Chunyan,et al."Construction of a high-density genetic map and fine QTL mapping for growth and nutritional traits of Crassostrea gigas".BMC GENOMICS 19(2018):15.

入库方式： OAI收割

来源：海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。