中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
Isolation and characterization of microsatellite markers for exploring introgressive hybridization between the Oratosquilla oratoria complex

文献类型:期刊论文

作者Cheng, Jiao1,2,3; Zhang, Nan1; Sha, Zhong-li1,2,3
刊名MOLECULAR BIOLOGY REPORTS
出版日期2018-10-01
卷号45期号:5页码:1499-1505
关键词Oratosquilla oratoria Transcriptome Microsatellite Hybridization
ISSN号0301-4851
DOI10.1007/s11033-018-4208-6
通讯作者Sha, Zhong-li(shazl@qdio.ac.cn)
英文摘要The Japanese mantis shrimp Oratosquilla oratoria is one of the most dominant stomatopod species found in coastal waters of the northwestern Pacific. We previously reported cryptic speciation and hybridization in this taxon by integrating mitochondrial and nuclear evidence. The present study aims at developing potential useful microsatellite markers for the O. oratoria complex through transcriptome sequencing, with a view to reveal the occurrence of hybridization. Of the 100 tested microsatellites, 55 were experimentally validated. 24 of these microsatellites were transferable across different species of the O. oratoria complex and showed polymorphic among individuals. The average number of alleles, observed and expected heterozygosity per locus was 6.125, 0.446 and 0.577 for the temperate species, and 6.083, 0.444 and 0.578 for the subtropical and tropical species. We also explore genetic differentiation and hybridization between O. oratoria cryptic species using these 24 microsatellite loci. The pairwise F (ST) values and phylogenetic tree indicated a strong genetic differentiation between the two cryptic species. In addition, Bayesian analysis provided evidence for the presence of hybridization between the O. oratoria complex. These markers provide valuable genomic resources for exploring introgressive hybridization and expanding understanding of evolution in the O. oratoria complex.
资助项目National Natural Science Foundation of China[31401955] ; Key Research Program of Frontier Sciences of the Chinese Academy of Sciences[QYZDB-SSW-DQC036]
WOS研究方向Biochemistry & Molecular Biology
语种英语
WOS记录号WOS:000444752900079
出版者SPRINGER
源URL[http://ir.qdio.ac.cn/handle/337002/160226]  
专题中国科学院海洋研究所
通讯作者Sha, Zhong-li
作者单位1.Chinese Acad Sci, Inst Oceanol, Qingdao 266071, Peoples R China
2.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714
Cheng, Jiao,Zhang, Nan,Sha, Zhong-li. Isolation and characterization of microsatellite markers for exploring introgressive hybridization between the Oratosquilla oratoria complex[J]. MOLECULAR BIOLOGY REPORTS,2018,45(5):1499-1505.
APA Cheng, Jiao,Zhang, Nan,&Sha, Zhong-li.(2018).Isolation and characterization of microsatellite markers for exploring introgressive hybridization between the Oratosquilla oratoria complex.MOLECULAR BIOLOGY REPORTS,45(5),1499-1505.
MLA Cheng, Jiao,et al."Isolation and characterization of microsatellite markers for exploring introgressive hybridization between the Oratosquilla oratoria complex".MOLECULAR BIOLOGY REPORTS 45.5(2018):1499-1505.

入库方式: OAI收割

来源:海洋研究所

浏览0
下载0
收藏0
其他版本

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。