SOX2 participates in spermatogenesis of Zhikong scallop Chlamysfarreri
文献类型：期刊论文
| 作者 | Liang, Shaoshuai1,2; Liu, Danwen1; Li, Xixi1; Wei, Maokai1; Yu, Xiaohan1; Li, Qi1; Ma, Huixin1; Zhang, Zhifeng1; Qin, Zhenkui1 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2019-01-11 |
| 卷号 | 9页码:13 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-018-35983-3 |
| 通讯作者 | Zhang, Zhifeng(zzfp107@ouc.edu.cn) ; Qin, Zhenkui(qinzk@ouc.edu.cn) |
| 英文摘要 | As an important transcription factor, SOX2 involves in embryogenesis, maintenance of stem cells and proliferation of primordial germ cell (PGC). However, little was known about its function in mature gonads. Herein, we investigated the SOX2 gene profiles in testis of scallop, Chlamys farreri. The level of C. farreri SOX2 (Cf-SOX2) mRNA increased gradually along with gonadal development and reached the peak at mature stage, and was located in all germ cells, including spermatogonia, spermatocytes, spermatids and spermatozoa. Knockdown of Cf-SOX2 using RNAi leaded to a mass of germ cells lost, and only a few spermatogonia retained in the nearly empty testicular acini after 21 days. TUNEL assay showed that apoptosis occurred in spermatocytes. Furthermore, transcriptome profiles of the testes were compared between Cf-SOX2 knockdown and normal scallops, 131,340 unigenes were obtained and 2,067 differential expression genes (DEGs) were identified. GO and KEGG analysis showed that most DEGs were related to cell apoptosis (casp2, casp3, casp8), cell proliferation (samd9, crebzf, iqsec1) and spermatogenesis (htt, tusc3, zmynd10, nipbl, mfge8), and enriched in p53, TNF and apoptosis pathways. Our study revealed Cf-SOX2 is essential in spermatogenesis and testis development of C. farreri and provided important clues for better understanding of SOX2 regulatory mechanisms in bivalve testis. |
| 资助项目 | Shandong Provincial Natural Science Foundation, China[ZR2016CQ24] ; National High Technology Research and Development Program of China[2012AA10A402] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000455470600022 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/160620]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Zhang, Zhifeng; Qin, Zhenkui |
| 作者单位 | 1.Ocean Univ China, Coll Marine Life Sci, Key Lab Marine Genet & Breeding, Minist Educ, Qingdao 266003, Peoples R China 2.Chinese Acad Sci, Inst Oceanol, Qingdao 266000, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liang, Shaoshuai,Liu, Danwen,Li, Xixi,et al. SOX2 participates in spermatogenesis of Zhikong scallop Chlamysfarreri[J]. SCIENTIFIC REPORTS,2019,9:13. |
| APA | Liang, Shaoshuai.,Liu, Danwen.,Li, Xixi.,Wei, Maokai.,Yu, Xiaohan.,...&Qin, Zhenkui.(2019).SOX2 participates in spermatogenesis of Zhikong scallop Chlamysfarreri.SCIENTIFIC REPORTS,9,13. |
| MLA | Liang, Shaoshuai,et al."SOX2 participates in spermatogenesis of Zhikong scallop Chlamysfarreri".SCIENTIFIC REPORTS 9(2019):13. |
入库方式： OAI收割
来源：海洋研究所
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