Isolation, identification, and characterization of diesel-oil-degrading bacterial strains indigenous to Changqing oil field, China
文献类型：期刊论文
| 作者 | Sun, Wuyang1; Ali, Imran1; Liu, Jiwei1; Dai, Min2; Cao, Wenrui3; Jiang, Mingyu3; Saren, Gaowa3; Yu, Xinke3; Peng, Changsheng1,2; Naz, Iffat4,5 |
| 刊名 | JOURNAL OF BASIC MICROBIOLOGY
 |
| 出版日期 | 2019-07-01 |
| 卷号 | 59期号:7页码:723-734 |
| 关键词 | Bacillus thuringiensis Bordetella bronchialis diesel-oil biodegradation Ochrobactrum anthropi |
| ISSN号 | 0233-111X |
| DOI | 10.1002/jobm.201800674 |
| 通讯作者 | Peng, Changsheng(cspeng@ouc.edu.cn) ; Naz, Iffat(iffatkhattak@yahoo.com) |
| 英文摘要 | In the present study, 12 indigenous diesel-oil-degrading bacteria were isolated from the petroleum-contaminated soils of the Changqing oil field (Xi'an, China). Measurement of the diesel-oil degradation rates of these strains by the gravimetric method revealed that they ranged from 42% to 66% within 2 weeks. The highest degradation rates were observed from strains CQ8-1 (66%), CQ8-2 (62.6%), and CQ11 (59%), which were identified as Bacillus thuringiensis, Ochrobactrum anthropi, and Bordetella bronchialis, respectively, based on their 16S rDNA sequences. Moreover, the physiological and biochemical properties of these three strains were analyzed by Gram staining, catalase, oxidase, and Voges-Proskauer tests. Transmission electron microscopy showed that all three strains were rod shaped with flagella. Gas chromatography and mass spectrometric analyses indicated that medium- and long-chain n-alkanes in diesel oil (C11-C29) were degraded to different degrees by B. thuringiensis, O. anthropi, and B. bronchialis, and the degradation rates gradually decreased as the carbon numbers increased. Overall, the results of this study indicate strains CQ8-1, CQ8-2, and CQ11 might be useful for environmentally friendly and cost-effective bioremediation of oil-contaminated soils. |
| 资助项目 | Shandong Province Key Research and Development Program[2016GSF115040] ; State Key Laboratory of Environmental Criteria and Risk Assessment[SKLECRA2013FP12] |
| WOS研究方向 | Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000474283500006 |
| 出版者 | WILEY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/161998]  |
| 专题 | 中国科学院海洋研究所 |
| 通讯作者 | Peng, Changsheng; Naz, Iffat |
| 作者单位 | 1.Ocean Univ China, Key Lab Marine Environm Sci & Ecol, Minist Educ, Qingdao, Shandong, Peoples R China 2.Zhaoqing Univ, Sch Environm & Chem Engn, Zhaoqing, Peoples R China 3.Chinese Acad Sci, Inst Oceanol, Qingdao, Shandong, Peoples R China 4.Qassim Univ, Dept Biol, Buraydah 51452, Saudi Arabia 5.Quaid I Azam Univ, Dept Microbiol, Islamabad, Pakistan |
| 推荐引用方式 GB/T 7714 | Sun, Wuyang,Ali, Imran,Liu, Jiwei,et al. Isolation, identification, and characterization of diesel-oil-degrading bacterial strains indigenous to Changqing oil field, China[J]. JOURNAL OF BASIC MICROBIOLOGY,2019,59(7):723-734. |
| APA | Sun, Wuyang.,Ali, Imran.,Liu, Jiwei.,Dai, Min.,Cao, Wenrui.,...&Naz, Iffat.(2019).Isolation, identification, and characterization of diesel-oil-degrading bacterial strains indigenous to Changqing oil field, China.JOURNAL OF BASIC MICROBIOLOGY,59(7),723-734. |
| MLA | Sun, Wuyang,et al."Isolation, identification, and characterization of diesel-oil-degrading bacterial strains indigenous to Changqing oil field, China".JOURNAL OF BASIC MICROBIOLOGY 59.7(2019):723-734. |
入库方式： OAI收割
来源：海洋研究所
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