Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens
文献类型：期刊论文
| 作者 | Zheng, Zhenbing1,2,3; Gao, Shan1,2; Huan, Li1,2 ; Wang, Guang-Ce1,2
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| 刊名 | PLANT JOURNAL
 |
| 出版日期 | 2018 |
| 卷号 | 93期号:1页码:119-130 |
| 关键词 | protonema seawater auxin cytokinin Physcomitrella patens |
| ISSN号 | 0960-7412 |
| DOI | 10.1111/tpj.13764 |
| 通讯作者 | Wang, Guang-Ce(gcwang@qdio.ac.cn) |
| 英文摘要 | Due to its highly efficient homologous recombination ability and unusual evolutionary position, the moss Physcomitrella patens has begun to attract more attention in genetic and evolutionary studies. Protonema, the filament stage of the gametophyte, is of great significance in P. patens protoplast isolation. Moreover, protonema is widely used in genetic engineering. However, difficulties in the induction and state maintenance of protonema restrict its wider application. In this work, protonema was induced efficiently in a diluted seawater medium, and the filamentous state was maintained without further cell differentiation. The developmental process of the protonema resumed, progressing to bud assembly and gametophore formation after transfer to freshwater medium. In addition, a transcriptome analysis showed that plant hormone signal transduction pathways were downregulated when protonema was grown in diluted seawater medium. Consistent with the transcriptome results, the protonema failed to respond to the addition of indole-3-acetic acid and 6-benzylaminopurine to the diluted seawater medium. Based on these results, we concluded that diluted seawater medium blocks the differentiation of protonema. This result could provide a novel insight to benefit future protonema production. |
| 资助项目 | Chinese Academy of Sciences[XDA11020404] ; National Natural Science Foundation of China[41376164] ; National Natural Science Foundation of China[41676157] ; National Natural Science Foundation of China[41606161] ; National Natural Science Foundation of China[41606159] ; National Natural Science Foundation of China[41476140] ; National Natural Science Foundation of China[41506172] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000418228000010 |
| 出版者 | WILEY |
| 源URL | [http://ir.qdio.ac.cn/handle/337002/157765]  |
| 专题 | 海洋研究所_实验海洋生物学重点实验室 |
| 通讯作者 | Wang, Guang-Ce |
| 作者单位 | 1.Chinese Acad Sci, Inst Oceanol, Key Lab Expt Marine Biol, Nanhai Rd 7, Qingdao 266071, Peoples R China 2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China 3.Univ Chinese Acad Sci, Coll Earth Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zheng, Zhenbing,Gao, Shan,Huan, Li,et al. Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens[J]. PLANT JOURNAL,2018,93(1):119-130. |
| APA | Zheng, Zhenbing,Gao, Shan,Huan, Li,&Wang, Guang-Ce.(2018).Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens.PLANT JOURNAL,93(1),119-130. |
| MLA | Zheng, Zhenbing,et al."Diluted seawater affects phytohormone receptors and maintains the protonema stage in Physcomitrella patens".PLANT JOURNAL 93.1(2018):119-130. |
入库方式： OAI收割
来源：海洋研究所
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